ISO 16000-36:2018
Indoor air — Part 36: Standard method for assessing the reduction rate of culturable airborne bacteria by air purifiers using a test chamber
This document specifies a method to evaluate the capacity of air purifiers to reduce the concentration of airborne culturable bacteria.
The test is applicable to air purifiers commonly used in single room spaces.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus and materials
5.1 Apparatus
5.2 Materials
5.2.1 Test bacteria
5.2.2 Culture media and reagents
6 Preparation of the stock cultures and working cultures of the test bacteria
6.1 Preparation and maintenance of stock culture
6.2 Preparation and maintenance of working cultures of the test bacteria on agar plates
6.3 Preparation of working culture suspensions
7 Procedures
7.1 General
7.2 Step 1 — Measurement of the concentration of culturable test bacteria, ci, without operating the air purifier
7.2.1 General
7.2.2 Preparation of the air purifier and the test chamber
7.2.3 Measurement of bacterial background concentration in the test chamber
7.2.4 Nebulizing test bacterial suspension
7.2.5 Measurement of the initial concentration of culturable bacteria inside the test chamber after nebulizing
7.2.6 Measurement of the concentration of culturable bacteria inside the test chamber after a defined time
7.2.7 Post-test actions
7.3 Step 2 — Measurement of the concentration of culturable test bacteria, ct, after operating the air purifier
8 Calculation and expression of results
8.1 Calculation of the concentration of airborne culturable bacteria
8.2 Conditions for a valid test
8.3 Reduction rate of bacteria
9 Test report
10 Quality assurance
Annex A (informative) Test chamber
Annex B (informative) Natural decay rate according to the operating mode of air purifier
Annex C (informative) Homogeneity of airborne culturable bacteria in the test chamber
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus and materials
5.1 Apparatus
5.2 Materials
5.2.1 Test bacteria
5.2.2 Culture media and reagents
6 Preparation of the stock cultures and working cultures of the test bacteria
6.1 Preparation and maintenance of stock culture
6.2 Preparation and maintenance of working cultures of the test bacteria on agar plates
6.3 Preparation of working culture suspensions
7 Procedures
7.1 General
7.2 Step 1 — Measurement of the concentration of culturable test bacteria, ci, without operating the air purifier
7.2.1 General
7.2.2 Preparation of the air purifier and the test chamber
7.2.3 Measurement of bacterial background concentration in the test chamber
7.2.4 Nebulizing test bacterial suspension
7.2.5 Measurement of the initial concentration of culturable bacteria inside the test chamber after nebulizing
7.2.6 Measurement of the concentration of culturable bacteria inside the test chamber after a defined time
7.2.7 Post-test actions
7.3 Step 2 — Measurement of the concentration of culturable test bacteria, ct, after operating the air purifier
8 Calculation and expression of results
8.1 Calculation of the concentration of airborne culturable bacteria
8.2 Conditions for a valid test
8.3 Reduction rate of bacteria
9 Test report
10 Quality assurance
Annex A (informative) Test chamber
Annex B (informative) Natural decay rate according to the operating mode of air purifier
Annex C (informative) Homogeneity of airborne culturable bacteria in the test chamber
Bibliography