ISO/TS 23459:2021
Nanotechnologies — Assessment of protein secondary structure during an interaction with nanomaterials using ultraviolet circular dichroism
This document specifies measurement protocols and test conditions to determine alterations to protein secondary structure induced by their interaction with nanomaterials using ultraviolet circular dichroism (UV-CD) spectroscopy.
This document does not apply to the characterization of conformational changes of disordered proteins.
| Get this standard | Prices exclude GST | |
|---|---|---|
| PDF ( Single user document) |
$256.00 NZD
|
|
| HardCopy |
$303.00 NZD
|
|
| Networkable PDF |
Price varies
|
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Nanomaterial protein interactions
6 Sample preparation
6.1 General
6.2 Desired properties of the UV-CD quartz cell
6.3 Preparation of protein solution
6.4 Instrumental setting condition
6.5 Recording UV-CD spectra procedure
6.5.1 General
6.5.2 Buffer
6.5.3 Protein sample
6.5.4 Stability of NP suspension in the protein solution
6.6 Preparation of protein-NPs conjugated suspension
6.7 UV-CD spectra measurement
6.8 Calculation of molar ellipticity
6.9 Data analysis
7 Test report
Annex A (informative) Typical UV-CD spectra of proteins
Annex B (informative) Literature survey on structural changes of NOAA and proteins
Annex C (informative) Description of buffers that can be used for protein solubility
Annex D (informative) Unit conversions in CD measurements
Annex E (informative) Calculating the concentration range of the sample
Annex F (informative) Methods for estimation of secondary structures of protein
Annex G (informative) Typical data of UV-CD used for estimation of secondary structures of protein
Bibliography
Keep me up-to-date
Sign up to receive updates when there are changes to this standard
Related Information
Similar Standards
-
BS EN 62341-1-1:2009
Organic light emitting diode (OLED) displays, Generic specifications
-
BS EN ISO 10801:2010
Nanotechnologies. Generation of metal nanoparticles for inhalation toxicity testing using the evaporation/condensation method
-
BS EN ISO 10808:2010
Nanotechnologies. Characterization of nanoparticles in inhalation exposure chambers for inhalation toxicity testing
-
BS EN ISO 17200:2020
Nanotechnology. Nanoparticles in powder form. Characteristics and measurements
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Nanomaterial protein interactions
6 Sample preparation
6.1 General
6.2 Desired properties of the UV-CD quartz cell
6.3 Preparation of protein solution
6.4 Instrumental setting condition
6.5 Recording UV-CD spectra procedure
6.5.1 General
6.5.2 Buffer
6.5.3 Protein sample
6.5.4 Stability of NP suspension in the protein solution
6.6 Preparation of protein-NPs conjugated suspension
6.7 UV-CD spectra measurement
6.8 Calculation of molar ellipticity
6.9 Data analysis
7 Test report
Annex A (informative) Typical UV-CD spectra of proteins
Annex B (informative) Literature survey on structural changes of NOAA and proteins
Annex C (informative) Description of buffers that can be used for protein solubility
Annex D (informative) Unit conversions in CD measurements
Annex E (informative) Calculating the concentration range of the sample
Annex F (informative) Methods for estimation of secondary structures of protein
Annex G (informative) Typical data of UV-CD used for estimation of secondary structures of protein
Bibliography