ISO/TS 22082:2020
Nanotechnologies — Assessment of nanomaterial toxicity using dechorionated zebrafish embryo
This document specifies a method for rapidly assessing nanomaterial toxicity (fish early life stage, 0 HPF to 120 HPF). It includes information on the importance of acellular chorion removal, detailed chorion removal procedures, and a complete protocol for the toxicity assessment of nanomaterials using dechorionated zebrafish embryos. The focus of this document is on testing nanomaterial toxicity.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Materials
6 Apparatus
6.1 Technical equipment
6.2 Analytical instruments
7 Procedures
7.1 Culture
7.1.1 Zebrafish strain
7.1.2 Feeding regime
7.1.3 Photoperiod
7.1.4 Temperature and pH
7.1.5 Tank material
7.2 Spawning stimulation
7.3 Dechorionation of embryos
7.3.1 Methods
7.3.2 Enzymatic dechorionation method
7.4 Preparation of nanomaterials stock solution
7.5 Testing concentrations
7.6 Dispersion of nanomaterials
7.7 Positive chemical: 3,4-dichloroaniline
7.8 Test methods
7.8.1 Water quality measurement
7.8.2 Preparation of dechorionated embryos
7.8.3 Exposure
7.8.4 Endpoints observation
7.9 Data analysis
8 Test report
8.1 Test procedure
8.2 Information to be included in the report
8.2.1 Test nanomaterials
8.2.2 Test species
8.2.3 Materials and apparatus
8.2.4 Maintenance
8.2.5 Test design
8.2.6 Test conditions
8.2.7 Results
Annex A (informative) Mechanical dechorionation method
Annex B (informative) Zebrafish embryo spawning procedures
Annex C (informative) Results validation
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Materials
6 Apparatus
6.1 Technical equipment
6.2 Analytical instruments
7 Procedures
7.1 Culture
7.1.1 Zebrafish strain
7.1.2 Feeding regime
7.1.3 Photoperiod
7.1.4 Temperature and pH
7.1.5 Tank material
7.2 Spawning stimulation
7.3 Dechorionation of embryos
7.3.1 Methods
7.3.2 Enzymatic dechorionation method
7.4 Preparation of nanomaterials stock solution
7.5 Testing concentrations
7.6 Dispersion of nanomaterials
7.7 Positive chemical: 3,4-dichloroaniline
7.8 Test methods
7.8.1 Water quality measurement
7.8.2 Preparation of dechorionated embryos
7.8.3 Exposure
7.8.4 Endpoints observation
7.9 Data analysis
8 Test report
8.1 Test procedure
8.2 Information to be included in the report
8.2.1 Test nanomaterials
8.2.2 Test species
8.2.3 Materials and apparatus
8.2.4 Maintenance
8.2.5 Test design
8.2.6 Test conditions
8.2.7 Results
Annex A (informative) Mechanical dechorionation method
Annex B (informative) Zebrafish embryo spawning procedures
Annex C (informative) Results validation
Bibliography