ISO 8199:2018
Water quality — General requirements and guidance for microbiological examinations by culture
This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate.
This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCR) methods.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 General measurement requirements
5.1 Uniformity of temperatures
5.2 Incubation times
5.3 Volumes and masses
6 Diluents and culture media
6.1 General
6.2 Quality requirements of ingredients
6.3 Diluents
7 Sterilization and decontamination
7.1 Sterilization of apparatus and glassware
7.2 Sterilization of consumables
7.3 Decontamination of glassware and materials after use
7.4 Waste management
8 Samples and sample handling
8.1 Sampling
8.2 Sample preparation
8.2.1 Waters and other aqueous matrices
8.2.2 Swabs
9 Enumeration (quantitative) methods
9.1 Inoculation of test portions in (or on) solid media
9.1.1 General
9.1.2 Pour plate technique
9.1.3 Spread plate technique
9.1.4 Membrane filtration technique
9.1.5 Incubation
9.1.6 Counting and confirmation from solid media
9.1.7 General guidance for calculation of results
9.1.8 Expression of results
9.2 Enumeration using a liquid medium
9.2.1 General
9.2.2 Procedure
9.2.3 Choice of inoculation system
9.2.4 Incubation
9.2.5 Interpretation of results
9.2.6 Uncertainty of test results
9.2.7 Determination of MPN values
10 Detection (qualitative) methods
10.1 General
10.2 Procedure
10.3 Uncertainty of test results
11 Performance characteristics of methods
12 Analytical quality control
12.1 General
12.2 Internal quality control
12.2.1 General
12.2.2 Process controls
12.3 External quality assessment
Annex A (informative) Criteria for the choice of technique
Annex B (informative) Confidence intervals for colony count technique and choice of method of calculation in special cases
Annex C (normative) Counting and calculations with two Petri dishes per dilution
Annex D (normative) Composition, preparation and performance testing of diluents
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 General measurement requirements
5.1 Uniformity of temperatures
5.2 Incubation times
5.3 Volumes and masses
6 Diluents and culture media
6.1 General
6.2 Quality requirements of ingredients
6.3 Diluents
7 Sterilization and decontamination
7.1 Sterilization of apparatus and glassware
7.2 Sterilization of consumables
7.3 Decontamination of glassware and materials after use
7.4 Waste management
8 Samples and sample handling
8.1 Sampling
8.2 Sample preparation
8.2.1 Waters and other aqueous matrices
8.2.2 Swabs
9 Enumeration (quantitative) methods
9.1 Inoculation of test portions in (or on) solid media
9.1.1 General
9.1.2 Pour plate technique
9.1.3 Spread plate technique
9.1.4 Membrane filtration technique
9.1.5 Incubation
9.1.6 Counting and confirmation from solid media
9.1.7 General guidance for calculation of results
9.1.8 Expression of results
9.2 Enumeration using a liquid medium
9.2.1 General
9.2.2 Procedure
9.2.3 Choice of inoculation system
9.2.4 Incubation
9.2.5 Interpretation of results
9.2.6 Uncertainty of test results
9.2.7 Determination of MPN values
10 Detection (qualitative) methods
10.1 General
10.2 Procedure
10.3 Uncertainty of test results
11 Performance characteristics of methods
12 Analytical quality control
12.1 General
12.2 Internal quality control
12.2.1 General
12.2.2 Process controls
12.3 External quality assessment
Annex A (informative) Criteria for the choice of technique
Annex B (informative) Confidence intervals for colony count technique and choice of method of calculation in special cases
Annex C (normative) Counting and calculations with two Petri dishes per dilution
Annex D (normative) Composition, preparation and performance testing of diluents
Bibliography