ISO 6647-1:2020
Rice — Determination of amylose content — Part 1: Spectrophotometric method with a defatting procedure by methanol and with calibration solutions of potato amylose and waxy rice amylopectin
This document specifies a reference method for the determination of the amylose content of milled rice, non-parboiled. The method is applicable to rice with an amylose mass fraction higher than 5 %.
This document can also be used for husked rice, maize, millet and other cereals if the extension of this scope has been validated by the user.
NOTE Amylose values determined with this document can be compared with PDO and PGI legislation.
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Procedure
8.1 Preparation of test sample
8.2 Test portion and preparation of the test solution
8.3 Preparation of the blank solution
8.4 Preparation of the calibration graph
8.4.1 Preparation of the set of calibration solutions
8.4.2 Colour development and spectrophotometric measurements
8.4.3 Plotting the calibration graph
8.5 Determination
9 Expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Determination of the quality of the potato amylose stock suspension
Annex B (informative) Results of an interlaboratory test
Annex C (informative) Example of a flow injection analyser (FIA) for the determination of amylose
Bibliography
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Procedure
8.1 Preparation of test sample
8.2 Test portion and preparation of the test solution
8.3 Preparation of the blank solution
8.4 Preparation of the calibration graph
8.4.1 Preparation of the set of calibration solutions
8.4.2 Colour development and spectrophotometric measurements
8.4.3 Plotting the calibration graph
8.5 Determination
9 Expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Determination of the quality of the potato amylose stock suspension
Annex B (informative) Results of an interlaboratory test
Annex C (informative) Example of a flow injection analyser (FIA) for the determination of amylose
Bibliography