ISO 23191:2020
Traditional Chinese medicine — Determination of selected Aconitum alkaloids by high-performance liquid chromatography (HPLC)
This document specifies methods for the determination of the selected Aconitum alkaloids, including aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine, benzoylhypaconine, yunaconitine, deacetyl-yunaconitine and crassicauline A.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Principle
6 Reagents
7 Apparatus
8 Sampling
9 Test procedures
9.1 General
9.2 HPLC-MS procedure
9.2.1 Reference solution
9.2.2 Test solution
9.2.3 Chromatographic conditions
9.2.4 HPLC-MS conditions
9.2.5 Determination
9.3 HPLC-DAD procedure
9.3.1 Reference solution
9.3.2 Test solution
9.3.3 Chromatographic conditions
9.3.4 Determination
10 Test report
Annex A (informative) Typical HPLC conditions
Annex B (informative) Typical chromatogram of the selected Aconitum alkaloids
Annex C (informative) Typical Aconitum herb used in traditional Chinese medicine
Annex D (informative) Reference values of national and regional limits of Aconitum alkaloids in Aconitum carmichaelii lateral root
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Principle
6 Reagents
7 Apparatus
8 Sampling
9 Test procedures
9.1 General
9.2 HPLC-MS procedure
9.2.1 Reference solution
9.2.2 Test solution
9.2.3 Chromatographic conditions
9.2.4 HPLC-MS conditions
9.2.5 Determination
9.3 HPLC-DAD procedure
9.3.1 Reference solution
9.3.2 Test solution
9.3.3 Chromatographic conditions
9.3.4 Determination
10 Test report
Annex A (informative) Typical HPLC conditions
Annex B (informative) Typical chromatogram of the selected Aconitum alkaloids
Annex C (informative) Typical Aconitum herb used in traditional Chinese medicine
Annex D (informative) Reference values of national and regional limits of Aconitum alkaloids in Aconitum carmichaelii lateral root
Bibliography