ISO 15714:2019
Method of evaluating the UV dose to airborne microorganisms transiting in-duct ultraviolet germicidal irradiation devices
This document describes a method in laboratory to assess the performance of ultraviolet germicidal irradiation (UVGI) devices which will be mounted in-duct in heating, ventilating and air-conditioning (HVAC) systems.
The method includes the detailed requirements for test rig, microorganisms, procedures, data calculation and result report to determine the UV dose to model microorganisms by an UVGI device at several airflow rates. By the testing results, the capacity of in-duct UVGI devices for air disinfection can be evaluated and compared reliably.
If the susceptibility constant of a given microorganism is known, the inactivation rate of that micro-organism by the tested UVGI devices can be further calculated.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms, definitions, symbols and abbreviated terms
3.1 Terms and definitions
3.2 Symbols and abbreviated terms
3.2.1 Symbols
3.2.2 Abbreviated terms
4 Configuration of the test rig
5 Test rig qualification
6 Preparation of test microorganisms
6.1 Test microorganisms
6.1.1 Serratia marcescens
6.1.2 Bacillus subtilis
6.1.3 Cladosporium sphaerospermum
6.2 Preparation of microbial suspensions
6.2.1 Acquisition of pure culture of test microorganisms
6.2.2 Cultivation and dispersion of the test microorganism
6.2.3 Dilution of the microbial suspensions
7 Testing procedure for an in-duct UVGI device
7.1 Determination of airflow rate, temperature and humidity
7.2 Production of the airborne test microorganism
7.3 Measurement of the test microorganism concentration without and with UV irradiation
7.3.1 Sampling procedure
7.3.2 Test microorganism sampling methods
7.3.3 Test microorganism culture and enumeration
7.4 Repeating the tests at other flow rates
7.5 Determination of the UV susceptibility of the test microorganism
8 Safety and environmental considerations
9 Calculation, evaluation and reporting
9.1 Determination of the inactivation rate of the test microorganism
9.2 Determination of the UV dose of the UVGI device
9.3 Evaluation of the UVGI capacity
9.4 Results reporting
Annex A (informative) Recipe of culture medium for the test microorganism
Annex B (informative) Method for determining the UV dose-response curve and susceptibility constant of a test microorganism in air
Annex C (informative) Susceptibility constants of some typical microorganisms in air by the literature
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms, definitions, symbols and abbreviated terms
3.1 Terms and definitions
3.2 Symbols and abbreviated terms
3.2.1 Symbols
3.2.2 Abbreviated terms
4 Configuration of the test rig
5 Test rig qualification
6 Preparation of test microorganisms
6.1 Test microorganisms
6.1.1 Serratia marcescens
6.1.2 Bacillus subtilis
6.1.3 Cladosporium sphaerospermum
6.2 Preparation of microbial suspensions
6.2.1 Acquisition of pure culture of test microorganisms
6.2.2 Cultivation and dispersion of the test microorganism
6.2.3 Dilution of the microbial suspensions
7 Testing procedure for an in-duct UVGI device
7.1 Determination of airflow rate, temperature and humidity
7.2 Production of the airborne test microorganism
7.3 Measurement of the test microorganism concentration without and with UV irradiation
7.3.1 Sampling procedure
7.3.2 Test microorganism sampling methods
7.3.3 Test microorganism culture and enumeration
7.4 Repeating the tests at other flow rates
7.5 Determination of the UV susceptibility of the test microorganism
8 Safety and environmental considerations
9 Calculation, evaluation and reporting
9.1 Determination of the inactivation rate of the test microorganism
9.2 Determination of the UV dose of the UVGI device
9.3 Evaluation of the UVGI capacity
9.4 Results reporting
Annex A (informative) Recipe of culture medium for the test microorganism
Annex B (informative) Method for determining the UV dose-response curve and susceptibility constant of a test microorganism in air
Annex C (informative) Susceptibility constants of some typical microorganisms in air by the literature
Bibliography