ISO 13885-1:2020
Gel permeation chromatography (GPC) — Part 1: Tetrahydrofuran (THF) as eluent
This document specifies the determination of the molar-mass distribution and the average molar mass values Mn (number average) and Mw (weight average) of polymers that are soluble in tetrahydrofuran (THF) by gel permeation chromatography (GPC).
NOTE Also known as size exclusion chromatography (SEC).
Even though the chromatograms obtained show good repeatability, it is possible that this method cannot be used with certain polymer types because of specific interactions (e.g. adsorption) within the sample/eluent/column system.
The conditions specified in this document are not applicable to the GPC analysis of polymer samples with Mw values greater than 106 g/mol and/or of polymers with elution limits outside the calibration range (see 7.6 and Annex C).
This document includes no correction method (e.g. for the elimination of peak broadening. If absolute molar-mass values are required, an absolute method (e.g. membrane osmometry for Mn or light scattering for Mw) can be used.
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
5.1 Eluent supply
5.2 Pump
5.3 Injection system
5.4 Separation columns
5.5 Column temperature control
5.6 Detector
6 Reagents
7 Calibration of the apparatus
7.1 General
7.2 Requirements for the calibration standards
7.3 Preparation of the calibration solutions for injection
7.4 Conditions for calibration runs
7.5 Measurement of elution volume
7.6 Plotting the calibration curve
8 Sampling
9 Preparation for the test
9.1 Preparation of the injection solution
9.2 Preparation of the apparatus
10 Analytical parameters
11 Data acquisition and evaluation
11.1 General
11.2 Calculation of the net chromatogram from the raw data
11.2.1 Determination of the baseline
11.2.2 Correction of the measured values and of the net chromatogram
11.2.3 Evaluation limits
11.3 Calculation of the average values
11.4 Calculation of the distribution curves
12 Precision
12.1 General
12.2 Repeatability
12.3 Reproducibility
13 Test report
13.1 General
13.2 General data on the equipment and settings
13.2.1 Data on the equipment used
13.2.2 Calibration
13.2.3 Evaluation
13.3 Special data on the sample
Annex A (informative) Conversion of experimental parameters for variant column sizes
Annex B (informative) Example of a data sheet for a polymer standard
Annex C (informative) Explanations
Bibliography
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
5.1 Eluent supply
5.2 Pump
5.3 Injection system
5.4 Separation columns
5.5 Column temperature control
5.6 Detector
6 Reagents
7 Calibration of the apparatus
7.1 General
7.2 Requirements for the calibration standards
7.3 Preparation of the calibration solutions for injection
7.4 Conditions for calibration runs
7.5 Measurement of elution volume
7.6 Plotting the calibration curve
8 Sampling
9 Preparation for the test
9.1 Preparation of the injection solution
9.2 Preparation of the apparatus
10 Analytical parameters
11 Data acquisition and evaluation
11.1 General
11.2 Calculation of the net chromatogram from the raw data
11.2.1 Determination of the baseline
11.2.2 Correction of the measured values and of the net chromatogram
11.2.3 Evaluation limits
11.3 Calculation of the average values
11.4 Calculation of the distribution curves
12 Precision
12.1 General
12.2 Repeatability
12.3 Reproducibility
13 Test report
13.1 General
13.2 General data on the equipment and settings
13.2.1 Data on the equipment used
13.2.2 Calibration
13.2.3 Evaluation
13.3 Special data on the sample
Annex A (informative) Conversion of experimental parameters for variant column sizes
Annex B (informative) Example of a data sheet for a polymer standard
Annex C (informative) Explanations
Bibliography