ISO 11816-1:2013
Milk and milk products — Determination of alkaline phosphatase activity — Part 1: Fluorimetric method for milk and milk-based drinks
ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.
The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/l.
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Section sec_1
Section sec_2
Section sec_2.1
Section sec_2.2
Section sec_3
Section sec_4
Section sec_4.1
Section sec_4.2
Section sec_4.3
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.4.3
Section sec_4.5
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.4
Section sec_5.5
Section sec_5.6
Section sec_5.7
Section sec_5.8
Section sec_6
Section sec_7
Section sec_7.1
Section sec_7.2
Section sec_7.2.1
Section sec_7.2.2
Section sec_7.2.3
Section sec_8
Section sec_8.1
Section sec_8.1.1
Section sec_8.1.2
Section sec_8.1.2.1
Section sec_8.1.2.2
Section sec_8.1.2.3
Section sec_8.1.3
Section sec_8.2
Section sec_8.3
Section sec_8.4
Section sec_8.5
Section sec_8.5.1
Section sec_8.5.1.1
Section sec_8.5.1.2
Section sec_8.5.2
Section sec_8.5.3
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.3
Section sec_10
Section sec_10.1
Section sec_10.2
Section sec_10.3
Section sec_11
Annex sec_A
Table tab_A.1
Table tab_A.2
Table tab_A.3
Table tab_A.4
Table tab_A.5
Reference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparations
7.1 Alkaline phosphatase-free milk
7.2 Preparation of test sample
8 Procedure
8.1 Verification of instrument performance
8.2 Reagent controls to test the suitability of ready-to-use working substrate (4.3)
8.3 Calibration
8.4 Determination
8.5 Test sample-related controls
9 Calculation and expression of results
9.1 Calibration ratio
9.2 Calculation
9.3 Expression of test results
10 Precision
10.1 Collaborative test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Collaborative trials
Bibliography
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Section sec_1
Section sec_2
Section sec_2.1
Section sec_2.2
Section sec_3
Section sec_4
Section sec_4.1
Section sec_4.2
Section sec_4.3
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.4.3
Section sec_4.5
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.4
Section sec_5.5
Section sec_5.6
Section sec_5.7
Section sec_5.8
Section sec_6
Section sec_7
Section sec_7.1
Section sec_7.2
Section sec_7.2.1
Section sec_7.2.2
Section sec_7.2.3
Section sec_8
Section sec_8.1
Section sec_8.1.1
Section sec_8.1.2
Section sec_8.1.2.1
Section sec_8.1.2.2
Section sec_8.1.2.3
Section sec_8.1.3
Section sec_8.2
Section sec_8.3
Section sec_8.4
Section sec_8.5
Section sec_8.5.1
Section sec_8.5.1.1
Section sec_8.5.1.2
Section sec_8.5.2
Section sec_8.5.3
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.3
Section sec_10
Section sec_10.1
Section sec_10.2
Section sec_10.3
Section sec_11
Annex sec_A
Table tab_A.1
Table tab_A.2
Table tab_A.3
Table tab_A.4
Table tab_A.5
Reference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparations
7.1 Alkaline phosphatase-free milk
7.2 Preparation of test sample
8 Procedure
8.1 Verification of instrument performance
8.2 Reagent controls to test the suitability of ready-to-use working substrate (4.3)
8.3 Calibration
8.4 Determination
8.5 Test sample-related controls
9 Calculation and expression of results
9.1 Calibration ratio
9.2 Calculation
9.3 Expression of test results
10 Precision
10.1 Collaborative test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Collaborative trials
Bibliography