ISO 11133:2014
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 11133:2014 defines terms related to quality assurance of culture media and specifies the requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as all kinds of water intended for consumption or used in food production. These requirements are applicable to all categories of culture media prepared for use in laboratories performing microbiological analyses.
ISO 11133:2014 also sets criteria and describes methods for the performance testing of culture media. It applies to producers such as:
- commercial bodies producing and/or distributing ready-to-use or semi-finished reconstituted or dehydrated media;
- non-commercial bodies supplying media to third parties;
- microbiological laboratories preparing culture media for their own use.
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Section sec_1
Section sec_2
Section sec_3
Section sec_3.1
Section sec_3.1.1
Section sec_3.1.2
Section sec_3.1.3
Section sec_3.2
Section sec_3.2.1
Section sec_3.2.2
Section sec_3.2.3
Section sec_3.2.4
Section sec_3.2.5
Section sec_3.2.6
Section sec_3.3
Section sec_3.3.1
Section sec_3.3.2.1
Section sec_3.3.2.2
Section sec_3.3.2.3
Section sec_3.3.3.1
Section sec_3.3.3.2
Section sec_3.3.4.1
Section sec_3.3.4.2
Section sec_3.3.4.3
Section sec_3.3.4.4
Section sec_3.3.4.5
Section sec_3.3.4.5.1
Section sec_3.3.4.5.2
Section sec_3.3.4.6
Section sec_3.3.4.6.1
Section sec_3.3.4.6.2
Section sec_3.3.4.6.3
Section sec_3.3.4.7
Section sec_3.3.4.8
Section sec_3.3.4.9
Section sec_3.3.4.10
Section sec_3.3.4.11
Section sec_3.3.4.12
Section sec_3.3.4.13
Section sec_3.3.5.1
Section sec_3.3.5.1.1
Section sec_3.3.5.1.2
Section sec_3.3.5.1.3
Section sec_3.3.5.2
Section sec_3.3.5.3
Section sec_3.4
Section sec_3.4.1
Section sec_3.4.2
Section sec_3.4.3
Section sec_3.4.4
Section sec_3.4.5
Section sec_3.4.6
Section sec_3.4.7
Section sec_4
Section sec_4.1
Section sec_4.1.1
Section sec_4.1.2
Section sec_4.2
Section sec_4.2.1
Section sec_4.2.2
Section sec_4.3
Section sec_4.3.1
Section sec_4.3.2
Section sec_4.3.3
Section sec_4.3.4
Section sec_4.3.5
Section sec_4.3.6
Section sec_4.3.7
Section sec_4.3.8
Section sec_4.3.8.1
Section sec_4.3.8.2
Section sec_4.3.8.3
Section sec_4.3.9
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.4.2.1
Section sec_4.4.2.2
Section sec_4.4.2.3
Section sec_4.5
Section sec_4.5.1
Section sec_4.5.2
Section sec_4.5.3
Section sec_4.5.4
Section sec_4.5.5
Section sec_4.6
Section sec_4.7
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.3.4
Section sec_5.3.5
Section sec_5.4
Section sec_5.4.1
Section sec_5.4.2
Section sec_5.4.2.1
Section sec_5.4.2.2
Section sec_5.4.2.3
Section sec_5.4.2.4
Section sec_5.4.2.5
Section sec_5.4.2.5.1
Section sec_5.4.2.5.1.1
Table tab_1
Section sec_5.4.2.5.1.2
Section sec_5.4.2.5.2
Section sec_5.4.2.5.3
Section sec_5.4.2.6
Section sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.3
Section sec_6.3.1
Section sec_6.3.2
Section sec_6.3.3
Section sec_6.4
Section sec_6.4.1
Section sec_6.4.2
Section sec_6.4.3
Section sec_6.4.4
Section sec_6.5
Section sec_6.6
Section sec_6.6.1
Section sec_6.6.2
Section sec_7
Section sec_7.1
Section sec_7.2
Section sec_7.2.1
Section sec_7.2.1.1
Section sec_7.2.1.2
Section sec_7.2.2
Section sec_7.2.2.1
Section sec_7.2.2.1.1
Section sec_7.2.2.1.2
Section sec_7.2.2.2
Section sec_7.3
Section sec_7.4
Section sec_7.4.1
Section sec_7.4.1.1
Section sec_7.4.1.2
Section sec_7.4.2
Section sec_7.4.3
Section sec_8
Section sec_8.1
Section sec_8.2
Section sec_8.2.1
Section sec_8.2.2
Section sec_8.2.3
Section sec_8.2.4
Section sec_8.3
Section sec_8.3.1
Section sec_8.3.2
Section sec_8.3.3
Section sec_8.4
Section sec_8.4.1
Section sec_8.4.2
Section sec_8.4.2.1
Section sec_8.4.2.2
Section sec_8.4.3
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.2.1
Section sec_9.2.1.1
Section sec_9.2.1.2
Section sec_9.2.1.3
Section sec_9.3
Section sec_9.3.1
Section sec_9.3.2
Section sec_9.3.2.1
Section sec_9.3.2.2
Section sec_9.3.3
Section sec_9.3.3.1
Section sec_9.3.3.2
Section sec_10
Section sec_10.1
Section sec_10.2
Annex sec_A
Annex sec_A.1
Annex sec_A.2
Annex sec_A.3
Annex sec_A.4
Annex sec_A.5
Annex sec_B
Annex sec_B.1
Figure fig_B.1
Annex sec_B.2
Figure fig_B.2
Annex sec_C
Annex sec_C.1
Annex sec_C.2
Table tab_c
Figure fig_C.1
Annex sec_C.3
Figure fig_C.2
Annex sec_C.4
Figure fig_C.3
Annex sec_C.5
Figure fig_C.4
Annex sec_D
Table tab_D.1
Annex sec_E
Table tab_E.2
Annex sec_F
Table tab_F.2
Annex sec_G
Annex sec_G.1
Annex sec_G.2
Annex sec_G.2.1
Annex sec_G.2.2
Annex sec_G.2.3
Table tab_G.1
Table tab_g
Figure fig_G.1
Annex sec_G.2.4
Annex sec_G.2.5
Annex sec_G.2.6
Table tab_G.2
Annex sec_H
Table tab_H.1
Annex sec_I
Annex sec_I.1
Annex sec_I.2
Annex sec_I.2.1
Annex sec_I.2.2
Annex sec_I.2.3
Figure fig_I.1
Annex sec_I.3
Annex sec_I.3.1
Annex sec_I.3.2
Annex sec_I.3.3
Figure fig_I.2
Annex sec_J
Annex sec_J.1
Annex sec_J.2
Annex sec_J.3
Table tab_J.1
Annex sec_J.4
Annex sec_J.4.1
Annex sec_J.4.2
Annex sec_J.4.3
Annex sec_J.4.4
Reference ref_1
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Reference ref_5
Reference ref_6
Reference ref_7
Reference ref_8
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Reference ref_53
Reference ref_54
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Reference ref_87
Table tab_E.1
Table tab_E.1___1
Table tab_E.1___2
Table tab_E.1___3
Table tab_E.1___4
Table tab_E.1___5
Table tab_E.1___6
Table tab_E.1___7
Table tab_E.1___8
Table tab_E.1___9
Table tab_E.1___10
Table tab_E.1___11
Table tab_E.1___12
Table tab_E.1___13
Table tab_E.1___14
Table tab_E.1___15
Table tab_E.1___16
Table tab_E.1___17
Table tab_F.1
Table tab_F.1___1
Table tab_F.1___2
Table tab_F.1___3
Table tab_F.1___4
Table tab_F.1___5
Table tab_F.1___6
Table tab_F.1___7
Table tab_F.1___8
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
3.1 General terms and definitions
3.2 Terminology of performance testing
3.3 Terminology of culture media
3.4 Terminology for test microorganisms
4 Quality assurance management
4.1 Documentation
4.2 Storage
4.3 Laboratory preparation of media
4.4 Storage and shelf-life of prepared media
4.5 Preparation for use
4.6 Incubation of solid media in Petri dishes
4.7 Disposal of media
5 Test organisms for performance testing
5.1 General
5.2 Selection of test organisms
5.3 Preservation and maintenance of test organisms
5.4 Microorganisms for performance testing
6 Quality control and performance testing of culture media
6.1 General requirements
6.2 Physical and chemical quality control
6.3 Microbiological quality control
6.4 General requirements for microbiological performance testing
6.5 Performance evaluation and interpretation of results
6.6 Confirmation media and reagents
7 Methods for performance testing of solid culture media
7.1 General
7.2 Methods for quantitative tests
7.3 Testing of culture media used for membrane filtration
7.4 Methods for qualitative tests
8 Methods for performance testing of liquid culture media
8.1 General
8.2 Quantitative tube method for performance testing of liquid enrichment media (dilution to extinction method)
8.3 Qualitative tube method for performance testing of selective liquid media
8.4 Qualitative single tube method (turbidity) for performance testing of liquid media
9 Methods for performance testing of diluents and transport media
9.1 General
9.2 Method for testing diluents
9.3 Method for testing transport media
10 Documentation of test results
10.1 Information provided by the manufacturer
10.2 Traceability
Annex A (informative) Designation of the components of culture media in International Standards on microbiological analysis of food, animal feed and water
Annex B (normative) Preparation of reference stock and working culture
Annex C (normative) Flow charts of methods for performance testing
Annex D (informative) Example of card for recording test results of culture media
Annex E (normative) Test microorganisms and performance criteria for culture media commonly used in food microbiology
Annex F (normative) Test microorganisms and performance criteria for culture media commonly used in water microbiology
Annex G (normative) Use of control charts to monitor quantitative testing of solid culture media
Annex H (informative) Quality assurance of culture media — Troubleshooting
Annex I (informative) Quantitative testing of liquid media
Annex J (normative) Definition of microbiological performance tests for standardized culture media
Bibliography
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Section sec_1
Section sec_2
Section sec_3
Section sec_3.1
Section sec_3.1.1
Section sec_3.1.2
Section sec_3.1.3
Section sec_3.2
Section sec_3.2.1
Section sec_3.2.2
Section sec_3.2.3
Section sec_3.2.4
Section sec_3.2.5
Section sec_3.2.6
Section sec_3.3
Section sec_3.3.1
Section sec_3.3.2.1
Section sec_3.3.2.2
Section sec_3.3.2.3
Section sec_3.3.3.1
Section sec_3.3.3.2
Section sec_3.3.4.1
Section sec_3.3.4.2
Section sec_3.3.4.3
Section sec_3.3.4.4
Section sec_3.3.4.5
Section sec_3.3.4.5.1
Section sec_3.3.4.5.2
Section sec_3.3.4.6
Section sec_3.3.4.6.1
Section sec_3.3.4.6.2
Section sec_3.3.4.6.3
Section sec_3.3.4.7
Section sec_3.3.4.8
Section sec_3.3.4.9
Section sec_3.3.4.10
Section sec_3.3.4.11
Section sec_3.3.4.12
Section sec_3.3.4.13
Section sec_3.3.5.1
Section sec_3.3.5.1.1
Section sec_3.3.5.1.2
Section sec_3.3.5.1.3
Section sec_3.3.5.2
Section sec_3.3.5.3
Section sec_3.4
Section sec_3.4.1
Section sec_3.4.2
Section sec_3.4.3
Section sec_3.4.4
Section sec_3.4.5
Section sec_3.4.6
Section sec_3.4.7
Section sec_4
Section sec_4.1
Section sec_4.1.1
Section sec_4.1.2
Section sec_4.2
Section sec_4.2.1
Section sec_4.2.2
Section sec_4.3
Section sec_4.3.1
Section sec_4.3.2
Section sec_4.3.3
Section sec_4.3.4
Section sec_4.3.5
Section sec_4.3.6
Section sec_4.3.7
Section sec_4.3.8
Section sec_4.3.8.1
Section sec_4.3.8.2
Section sec_4.3.8.3
Section sec_4.3.9
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.4.2.1
Section sec_4.4.2.2
Section sec_4.4.2.3
Section sec_4.5
Section sec_4.5.1
Section sec_4.5.2
Section sec_4.5.3
Section sec_4.5.4
Section sec_4.5.5
Section sec_4.6
Section sec_4.7
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.3.4
Section sec_5.3.5
Section sec_5.4
Section sec_5.4.1
Section sec_5.4.2
Section sec_5.4.2.1
Section sec_5.4.2.2
Section sec_5.4.2.3
Section sec_5.4.2.4
Section sec_5.4.2.5
Section sec_5.4.2.5.1
Section sec_5.4.2.5.1.1
Table tab_1
Section sec_5.4.2.5.1.2
Section sec_5.4.2.5.2
Section sec_5.4.2.5.3
Section sec_5.4.2.6
Section sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.3
Section sec_6.3.1
Section sec_6.3.2
Section sec_6.3.3
Section sec_6.4
Section sec_6.4.1
Section sec_6.4.2
Section sec_6.4.3
Section sec_6.4.4
Section sec_6.5
Section sec_6.6
Section sec_6.6.1
Section sec_6.6.2
Section sec_7
Section sec_7.1
Section sec_7.2
Section sec_7.2.1
Section sec_7.2.1.1
Section sec_7.2.1.2
Section sec_7.2.2
Section sec_7.2.2.1
Section sec_7.2.2.1.1
Section sec_7.2.2.1.2
Section sec_7.2.2.2
Section sec_7.3
Section sec_7.4
Section sec_7.4.1
Section sec_7.4.1.1
Section sec_7.4.1.2
Section sec_7.4.2
Section sec_7.4.3
Section sec_8
Section sec_8.1
Section sec_8.2
Section sec_8.2.1
Section sec_8.2.2
Section sec_8.2.3
Section sec_8.2.4
Section sec_8.3
Section sec_8.3.1
Section sec_8.3.2
Section sec_8.3.3
Section sec_8.4
Section sec_8.4.1
Section sec_8.4.2
Section sec_8.4.2.1
Section sec_8.4.2.2
Section sec_8.4.3
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.2.1
Section sec_9.2.1.1
Section sec_9.2.1.2
Section sec_9.2.1.3
Section sec_9.3
Section sec_9.3.1
Section sec_9.3.2
Section sec_9.3.2.1
Section sec_9.3.2.2
Section sec_9.3.3
Section sec_9.3.3.1
Section sec_9.3.3.2
Section sec_10
Section sec_10.1
Section sec_10.2
Annex sec_A
Annex sec_A.1
Annex sec_A.2
Annex sec_A.3
Annex sec_A.4
Annex sec_A.5
Annex sec_B
Annex sec_B.1
Figure fig_B.1
Annex sec_B.2
Figure fig_B.2
Annex sec_C
Annex sec_C.1
Annex sec_C.2
Table tab_c
Figure fig_C.1
Annex sec_C.3
Figure fig_C.2
Annex sec_C.4
Figure fig_C.3
Annex sec_C.5
Figure fig_C.4
Annex sec_D
Table tab_D.1
Annex sec_E
Table tab_E.2
Annex sec_F
Table tab_F.2
Annex sec_G
Annex sec_G.1
Annex sec_G.2
Annex sec_G.2.1
Annex sec_G.2.2
Annex sec_G.2.3
Table tab_G.1
Table tab_g
Figure fig_G.1
Annex sec_G.2.4
Annex sec_G.2.5
Annex sec_G.2.6
Table tab_G.2
Annex sec_H
Table tab_H.1
Annex sec_I
Annex sec_I.1
Annex sec_I.2
Annex sec_I.2.1
Annex sec_I.2.2
Annex sec_I.2.3
Figure fig_I.1
Annex sec_I.3
Annex sec_I.3.1
Annex sec_I.3.2
Annex sec_I.3.3
Figure fig_I.2
Annex sec_J
Annex sec_J.1
Annex sec_J.2
Annex sec_J.3
Table tab_J.1
Annex sec_J.4
Annex sec_J.4.1
Annex sec_J.4.2
Annex sec_J.4.3
Annex sec_J.4.4
Reference ref_1
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Table tab_E.1
Table tab_E.1___1
Table tab_E.1___2
Table tab_E.1___3
Table tab_E.1___4
Table tab_E.1___5
Table tab_E.1___6
Table tab_E.1___7
Table tab_E.1___8
Table tab_E.1___9
Table tab_E.1___10
Table tab_E.1___11
Table tab_E.1___12
Table tab_E.1___13
Table tab_E.1___14
Table tab_E.1___15
Table tab_E.1___16
Table tab_E.1___17
Table tab_F.1
Table tab_F.1___1
Table tab_F.1___2
Table tab_F.1___3
Table tab_F.1___4
Table tab_F.1___5
Table tab_F.1___6
Table tab_F.1___7
Table tab_F.1___8
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
3.1 General terms and definitions
3.2 Terminology of performance testing
3.3 Terminology of culture media
3.4 Terminology for test microorganisms
4 Quality assurance management
4.1 Documentation
4.2 Storage
4.3 Laboratory preparation of media
4.4 Storage and shelf-life of prepared media
4.5 Preparation for use
4.6 Incubation of solid media in Petri dishes
4.7 Disposal of media
5 Test organisms for performance testing
5.1 General
5.2 Selection of test organisms
5.3 Preservation and maintenance of test organisms
5.4 Microorganisms for performance testing
6 Quality control and performance testing of culture media
6.1 General requirements
6.2 Physical and chemical quality control
6.3 Microbiological quality control
6.4 General requirements for microbiological performance testing
6.5 Performance evaluation and interpretation of results
6.6 Confirmation media and reagents
7 Methods for performance testing of solid culture media
7.1 General
7.2 Methods for quantitative tests
7.3 Testing of culture media used for membrane filtration
7.4 Methods for qualitative tests
8 Methods for performance testing of liquid culture media
8.1 General
8.2 Quantitative tube method for performance testing of liquid enrichment media (dilution to extinction method)
8.3 Qualitative tube method for performance testing of selective liquid media
8.4 Qualitative single tube method (turbidity) for performance testing of liquid media
9 Methods for performance testing of diluents and transport media
9.1 General
9.2 Method for testing diluents
9.3 Method for testing transport media
10 Documentation of test results
10.1 Information provided by the manufacturer
10.2 Traceability
Annex A (informative) Designation of the components of culture media in International Standards on microbiological analysis of food, animal feed and water
Annex B (normative) Preparation of reference stock and working culture
Annex C (normative) Flow charts of methods for performance testing
Annex D (informative) Example of card for recording test results of culture media
Annex E (normative) Test microorganisms and performance criteria for culture media commonly used in food microbiology
Annex F (normative) Test microorganisms and performance criteria for culture media commonly used in water microbiology
Annex G (normative) Use of control charts to monitor quantitative testing of solid culture media
Annex H (informative) Quality assurance of culture media — Troubleshooting
Annex I (informative) Quantitative testing of liquid media
Annex J (normative) Definition of microbiological performance tests for standardized culture media
Bibliography