AS/NZS 4276.15:2014
Water microbiology – Method 15: Examination for Vibrio cholerae
This standard covers the detection of detection of V. cholerae in water using conventional culture-based techniques. The enumeration of this microorganism is not normally carried out because its presence, irrespective of numbers, is significant from a public health point of view.
The V. cholerae species is composed of numerous O-serotypes. Toxin producing strains of the O1 serotype are responsible for epidemic cholera. However, another serotype, O139, has relatively recently been identified as an epidemic producing strain. Other strains belonging to different serotypes may cause sporadic cases of diarrhoeal illness. This method will detect all strains of V. cholerae and allow identification of the cholera epidemic producing O1 and O139 serotypes.
In 2025, the P4828 Rejoining of Microbiology Standards committee was formed to consider this standard’s suitability (and the suitability of other Australian water microbiology standards) for rejoining as Australian/New Zealand standards.
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AS/NZS 4276.15:2014
SA/SNZ - Cover page
Preface
1 Scope
2 Referenced documents
3 Definition
4 Principle
5 Culture media and reagents
5.1 Culture media
5.2 Reagents
6 Apparatus
6.1 Apparatus and materials for membrane filtration
6.2 Incubators or water baths
7 Reference cultures
7.1 Positive culture
7.2 Use of reference culture
8 Samples
8.1 Laboratory sample
8.2 Sample volume
8.3 Concentration techniques
8.3.1 Moore swab
8.3.2 Membrane filtration
9 Procedure
9.1 Enrichment
9.2 Selective agar culture
9.3 Presumptive identification of Vibrio cholerae
9.4 Confirmation of Vibrio cholerae
9.4.1 Phenotypic identification and serological characterization
9.4.2 Genotypic tests
10 Test report
11 Bibliography
Appendix A (informative) Flow chart for V. cholerae testing
Appendix B (informative) Culture media
Appendix C (informative) The use of diatomaceous earth (‘filter aid’) for facilitating the filtration of turbid water samples
Appendix D (informative) Performance of conventional tests requiring addition of reagents
Appendix E (informative) Detection of the genes for Vibrio cholerae toxin, serotypes O1 and O139, and haemolysin by multiplex polymerase chain reaction (PCR)
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AS/NZS 4276.15:2014
SA/SNZ - Cover page
Preface
1 Scope
2 Referenced documents
3 Definition
4 Principle
5 Culture media and reagents
5.1 Culture media
5.2 Reagents
6 Apparatus
6.1 Apparatus and materials for membrane filtration
6.2 Incubators or water baths
7 Reference cultures
7.1 Positive culture
7.2 Use of reference culture
8 Samples
8.1 Laboratory sample
8.2 Sample volume
8.3 Concentration techniques
8.3.1 Moore swab
8.3.2 Membrane filtration
9 Procedure
9.1 Enrichment
9.2 Selective agar culture
9.3 Presumptive identification of Vibrio cholerae
9.4 Confirmation of Vibrio cholerae
9.4.1 Phenotypic identification and serological characterization
9.4.2 Genotypic tests
10 Test report
11 Bibliography
Appendix A (informative) Flow chart for V. cholerae testing
Appendix B (informative) Culture media
Appendix C (informative) The use of diatomaceous earth (‘filter aid’) for facilitating the filtration of turbid water samples
Appendix D (informative) Performance of conventional tests requiring addition of reagents
Appendix E (informative) Detection of the genes for Vibrio cholerae toxin, serotypes O1 and O139, and haemolysin by multiplex polymerase chain reaction (PCR)
Amendment control sheet
Previous versions
AS/NZS 4276.15:2014
Online Library subscription information
Online library subscriptions provide a cost-effective solution for organisations of any size to access the latest standards, offering 24/7 access to a personalised catalogue. The service ensures subscribers have have the most up-to-date version of selected standards, incluing automatic updates for any amendments or versions. Pricing is dependent on the chosen standards and the number of concurrent users, with discounts applied from the standards PDF price. For customised subscriptions or further inquiries, contact the Online Library team.