AS/NZS 4276.15:1999
Water microbiology - Method 15: Examination for Vibrio cholerae
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AS/NZS 4276.15:1999 WATER MICROBIOLOGY - EXAMINATION FOR VIBRIO CHOLERAE
PREFACE
METHOD
1 SCOPE
2 PRINCIPLE
3 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
3.1 Culture media
3.2 Reagents
3.3 Reference cultures
4 SAMPLES
4.1 Sample volume
4.2 Concentration techniques
5 PROCEDURE
5.1 Enrichment
5.2 Selective agar cultures
5.3 Presumptive identification of Vibrio cholerae
5.4 Confirmation of Vibrio cholerae
6 TEST REPORT
7 ENUMERATION PROCEDURES
8 BIBLIOGRAPHY
APPENDIX A - FLOW CHART FOR TESTING
APPENDIX B - CULTURE MEDIA
B1 SCOPE
B2 0%, 3%, 8% NaCl TRYPTONE WATER
B3 ALKALINE PEPTONE WATER
B4 BUFFERED GLUCOSE BROTH
B5 CARBOHYDRATE FERMENTATION BROTHS
B5.1 Preparation of complete medium
B5.2 Fermentation Broth base
B5.3 Andrade's indicator
B6 DECARBOXYLASE BROTH (Moeller's)
B6.1 Preparation of complete medium
B6.2 Decarboxylase broth base (Moeller's)
B7 NITRATE REDUCTION BROTH
B8 THIOSULFATE CITRATE BILE SALTS SUCROSE AGAR
B9 TRYPTONE WATER
B10 ONPG BROTH
B10.1 Preparation of complete medium
B10.2 Preparation of ONPG solution
APPENDIX C - PERFORMANCE OF CONVENTIONAL TESTS REQUIRING ADDITION OF REAGENTS
C1 INDOLE TEST
C2 VOGES-PROSKAUER TEST (FOR THE PRODUCTION OF ACETOIN)
C3 NITRATE REDUCTION TEST (Barritt)
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Related Information
Australia New Zealand Food Standards Code Standard 1.6.1
Ministry: Food Standards Australia New Zealand
Section: 4.1(b)
Citation context:...for packaged water, packaged ice or mineral water - the relevant method prescribed by Australian New Zealand Standard AS/NZS 4276.
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AS/NZS 4276.15:1999 WATER MICROBIOLOGY - EXAMINATION FOR VIBRIO CHOLERAE
PREFACE
METHOD
1 SCOPE
2 PRINCIPLE
3 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
3.1 Culture media
3.2 Reagents
3.3 Reference cultures
4 SAMPLES
4.1 Sample volume
4.2 Concentration techniques
5 PROCEDURE
5.1 Enrichment
5.2 Selective agar cultures
5.3 Presumptive identification of Vibrio cholerae
5.4 Confirmation of Vibrio cholerae
6 TEST REPORT
7 ENUMERATION PROCEDURES
8 BIBLIOGRAPHY
APPENDIX A - FLOW CHART FOR TESTING
APPENDIX B - CULTURE MEDIA
B1 SCOPE
B2 0%, 3%, 8% NaCl TRYPTONE WATER
B3 ALKALINE PEPTONE WATER
B4 BUFFERED GLUCOSE BROTH
B5 CARBOHYDRATE FERMENTATION BROTHS
B5.1 Preparation of complete medium
B5.2 Fermentation Broth base
B5.3 Andrade's indicator
B6 DECARBOXYLASE BROTH (Moeller's)
B6.1 Preparation of complete medium
B6.2 Decarboxylase broth base (Moeller's)
B7 NITRATE REDUCTION BROTH
B8 THIOSULFATE CITRATE BILE SALTS SUCROSE AGAR
B9 TRYPTONE WATER
B10 ONPG BROTH
B10.1 Preparation of complete medium
B10.2 Preparation of ONPG solution
APPENDIX C - PERFORMANCE OF CONVENTIONAL TESTS REQUIRING ADDITION OF REAGENTS
C1 INDOLE TEST
C2 VOGES-PROSKAUER TEST (FOR THE PRODUCTION OF ACETOIN)
C3 NITRATE REDUCTION TEST (Barritt)
AS/NZS 4276.15:1999
Online Library subscription information
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