ISO 24443:2012
Determination of sunscreen UVA photoprotection in vitro
ISO 24443:2012 specifies an in vitro procedure to characterize the UVA protection of sunscreen products. Specifications are given to enable determination of the spectral absorbance characteristics of UVA protection in a reproducible manner.
In order to determine relevant UVA protection parameters, the method has been created to provide a UV spectral absorbance curve from which a number of calculations and evaluations can be undertaken. This method relies on the use of in vivo SPF results for scaling the UV absorbance curve.
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Bibliography
Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Apparatus
4.1 UV spectrophotometer specifications
4.2 Calibration of the UV spectrophotometer
4.3 Calibration of the UV exposure source
4.4 Monitoring of the UV exposure source
4.5 Calibration of the UVA radiometer used to monitor the test sample irradiation
4.6 Substrate/plate
5 Test method
5.1 Outline of the test procedure
5.2 Equipment calibration and validation of test plates
5.3 Absorption measurements through the plate
5.4 Sample application
5.5 Absorbance measurements of the product-treated plate
5.6 Number of determinations
5.7 Determination of initial calculated SPF (SPFin vitro), “C” value, initial UVAPF(UVAPF0), and UV exposure dose
5.8 UV exposure
5.9 Measurement of final adjusted absorbance spectrum
5.10 Calculation of UVAPF of plates after UV exposure of the sample
6 Procedure using the spreadsheet in this International Standard
7 Reference sunscreen formula S2
8 Test report
Annex A (normative) Calibration of UV spectrophotometer and plate transmission test
Annex B (normative) Radiometer calibration to spectroradiometric irradiance procedure
Annex C (normative) Computation values: PPD and erythema action spectra and UVA and UV-SSR spectral irradiances
Annex D (normative) PMMA test plate surface specifications
Annex E (normative) UVA reference sunscreen S2
Annex F (informative) Statistical calculations
Bibliography
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Bibliography
Foreword
1 Scope
2 Terms and definitions
3 Principle
4 Apparatus
4.1 UV spectrophotometer specifications
4.2 Calibration of the UV spectrophotometer
4.3 Calibration of the UV exposure source
4.4 Monitoring of the UV exposure source
4.5 Calibration of the UVA radiometer used to monitor the test sample irradiation
4.6 Substrate/plate
5 Test method
5.1 Outline of the test procedure
5.2 Equipment calibration and validation of test plates
5.3 Absorption measurements through the plate
5.4 Sample application
5.5 Absorbance measurements of the product-treated plate
5.6 Number of determinations
5.7 Determination of initial calculated SPF (SPFin vitro), “C” value, initial UVAPF(UVAPF0), and UV exposure dose
5.8 UV exposure
5.9 Measurement of final adjusted absorbance spectrum
5.10 Calculation of UVAPF of plates after UV exposure of the sample
6 Procedure using the spreadsheet in this International Standard
7 Reference sunscreen formula S2
8 Test report
Annex A (normative) Calibration of UV spectrophotometer and plate transmission test
Annex B (normative) Radiometer calibration to spectroradiometric irradiance procedure
Annex C (normative) Computation values: PPD and erythema action spectra and UVA and UV-SSR spectral irradiances
Annex D (normative) PMMA test plate surface specifications
Annex E (normative) UVA reference sunscreen S2
Annex F (informative) Statistical calculations
Bibliography