ISO 17604:2015
Microbiology of the food chain — Carcass sampling for microbiological analysis
ISO 17604:2015 specifies sampling methods for the detection and enumeration of microorganisms on the surface of carcasses or parts of carcasses of slaughtered meat animals. The microbiological sampling can be carried out as part of
- process hygiene control (to validate and or verify process control, e.g. total counts and Enterobacteriaceae) in slaughter establishments for large mammals, poultry, and game,
- risk-based assurance systems for product safety, and
- monitoring or surveillance programmes for the prevalence and/or numbers of pathogenic microorganisms.
This International Standard includes the use of excision and swabbing techniques depending on the reason for sample collection. It also includes the use of carcass rinsing for the examination of carcasses of poultry and some small animals. Annex A shows sampling sites on the carcasses of various animal species.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 General principles
5 Sampling plans
6 Sampling points on the production line
7 Sampling sites on carcasses
7.1 Large mammals
7.2 Poultry, small mammals, and other small animals
7.3 Game
8 Sampling techniques
8.1 General
8.2 Excision methods
8.2.1 Cork borer method
8.2.2 Template excision method
8.2.3 Skin sampling
8.3 Swabbing methods
8.3.1 Wet and dry stick swab method
8.3.2 Sponge, tampon swab, and small cloth method
8.4 Carcass rinsing method
8.4.1 Reagent
8.4.2 Equipment
8.4.3 Collection of samples
9 Storage and transport of samples
10 Microbiological examination of samples
10.1 General
10.2 Initial preparation of samples
10.2.1 Swabbing methods
10.2.2 Excised samples
10.2.3 Carcass rinse samples
10.3 Enumeration and detection of microorganisms
Annex A (informative) Sampling sites
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 General principles
5 Sampling plans
6 Sampling points on the production line
7 Sampling sites on carcasses
7.1 Large mammals
7.2 Poultry, small mammals, and other small animals
7.3 Game
8 Sampling techniques
8.1 General
8.2 Excision methods
8.2.1 Cork borer method
8.2.2 Template excision method
8.2.3 Skin sampling
8.3 Swabbing methods
8.3.1 Wet and dry stick swab method
8.3.2 Sponge, tampon swab, and small cloth method
8.4 Carcass rinsing method
8.4.1 Reagent
8.4.2 Equipment
8.4.3 Collection of samples
9 Storage and transport of samples
10 Microbiological examination of samples
10.1 General
10.2 Initial preparation of samples
10.2.1 Swabbing methods
10.2.2 Excised samples
10.2.3 Carcass rinse samples
10.3 Enumeration and detection of microorganisms
Annex A (informative) Sampling sites
Bibliography