ISO 11290-1:2017
Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method
ISO 11290-1:2017 specifies a horizontal method for
- the detection of L. monocytogenes, and
- the detection of Listeria spp. (including L. monocytogenes).
ISO 11290-1:2017 is applicable to
- products intended for human consumption and for the feeding of animals, and
- environmental samples in the area of food production and food handling.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth)
4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.4 Plating out and identification
4.5 Confirmation
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment
9.3 Secondary enrichment
9.4 Plating out and identification
9.4.1 General
9.4.2 Agar Listeria according to Ottaviani and Agosti
9.4.3 Second selective medium
9.5 Confirmation of Listeria monocytogenes or Listeria spp.
9.5.1 Selection of colonies for confirmation
9.5.2 Confirmation of L. monocytogenes
9.5.3 Confirmation of Listeria spp.
9.6 Interpretation of morphological and physiological properties and of the biochemical reactions
9.7 Additional characterization of isolated strains (optional)
10 Expression of results
11 Performance characteristics of the method
11.1 Method validation studies
11.2 Sensitivity
11.3 Specificity
11.4 Level of detection (LOD50)
12 Test report
13 Quality assurance
Annex A (normative) Diagram of procedure
Annex B (normative) Composition and preparation of culture media and reagents
Annex C (informative) Distinction of Listeria spp. from other genera
Annex D (informative) Reactions for the identification of Listeria species
Annex E (informative) Listeria selective agars
Annex F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth)
4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.4 Plating out and identification
4.5 Confirmation
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment
9.3 Secondary enrichment
9.4 Plating out and identification
9.4.1 General
9.4.2 Agar Listeria according to Ottaviani and Agosti
9.4.3 Second selective medium
9.5 Confirmation of Listeria monocytogenes or Listeria spp.
9.5.1 Selection of colonies for confirmation
9.5.2 Confirmation of L. monocytogenes
9.5.3 Confirmation of Listeria spp.
9.6 Interpretation of morphological and physiological properties and of the biochemical reactions
9.7 Additional characterization of isolated strains (optional)
10 Expression of results
11 Performance characteristics of the method
11.1 Method validation studies
11.2 Sensitivity
11.3 Specificity
11.4 Level of detection (LOD50)
12 Test report
13 Quality assurance
Annex A (normative) Diagram of procedure
Annex B (normative) Composition and preparation of culture media and reagents
Annex C (informative) Distinction of Listeria spp. from other genera
Annex D (informative) Reactions for the identification of Listeria species
Annex E (informative) Listeria selective agars
Annex F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes
Bibliography