ISO 23190:2021
Traditional Chinese medicine — Determination of aristolochic acids in natural products by high-performance liquid chromatography (HPLC)
This document specifies a method for using high-performance liquid chromatography (HPLC) to determine the presence of aristolochic acid I in natural products.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Principle
6 Reagents
7 Apparatus
8 Test method
8.1 General principle
8.2 Caution and safety considerations
8.3 Stock solution of standard
8.4 HPLC condition
8.4.1 Chromatographic system and system suitability
8.4.2 Standard solution
8.4.3 Test solution I
8.4.4 Test solution II
8.4.5 Procedure
8.5 Method validation
8.6 Evaluation of qualitative results
9 Sampling and preservation of samples
9.1 Sampling
10 Test report
Annex A (informative) Typical HPLC conditions
Annex B (informative) Qualitative determination of aristolochic acid I by LC-MS
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Principle
6 Reagents
7 Apparatus
8 Test method
8.1 General principle
8.2 Caution and safety considerations
8.3 Stock solution of standard
8.4 HPLC condition
8.4.1 Chromatographic system and system suitability
8.4.2 Standard solution
8.4.3 Test solution I
8.4.4 Test solution II
8.4.5 Procedure
8.5 Method validation
8.6 Evaluation of qualitative results
9 Sampling and preservation of samples
9.1 Sampling
10 Test report
Annex A (informative) Typical HPLC conditions
Annex B (informative) Qualitative determination of aristolochic acid I by LC-MS
Bibliography