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ISO 23036-2:2021

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Microbiology of the food chain — Methods for the detection of Anisakidae L3 larvae in fish and fishery products — Part 2: Artificial digestion method

This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.

The artificial digestion method[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.

This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.

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