ISO 21528-2:2017
Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 2: Colony-count technique
ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.
The most probable number (MPN) technique, as included in ISO 21528‑1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Preparation of initial suspension and decimal dilutions
4.2 Isolation and selection for confirmation
4.3 Confirmation
4.4 Calculation
5 Diluent, culture media and reagent
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion, initial suspension and dilutions
9.3 Inoculation and incubation
9.4 Counting and selection of colonies for confirmation
9.5 Subculturing selected colonies
9.6 Biochemical confirmation tests
9.6.1 Oxidase reaction
9.6.2 Fermentation test
9.6.3 Interpretation of biochemical tests
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Repeatability limit
11.3 Reproducibility limit
12 Test report
13 Quality assurance
Annex A (normative) Culture media and reagents
Annex B (informative) Method validation studies and performance characteristics
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Preparation of initial suspension and decimal dilutions
4.2 Isolation and selection for confirmation
4.3 Confirmation
4.4 Calculation
5 Diluent, culture media and reagent
6 Equipment and consumables
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion, initial suspension and dilutions
9.3 Inoculation and incubation
9.4 Counting and selection of colonies for confirmation
9.5 Subculturing selected colonies
9.6 Biochemical confirmation tests
9.6.1 Oxidase reaction
9.6.2 Fermentation test
9.6.3 Interpretation of biochemical tests
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Repeatability limit
11.3 Reproducibility limit
12 Test report
13 Quality assurance
Annex A (normative) Culture media and reagents
Annex B (informative) Method validation studies and performance characteristics
Bibliography