ISO 18862:2016
Coffee and coffee products — Determination of acrylamide — Methods using HPLC-MS/MS and GC-MS after derivatization
ISO 18862:2016 specifies methods for the determination of acrylamide in coffee and coffee products by extraction with water, clean-up by solid-phase extraction and determination by HPLC-MS/MS and GC-MS. It was validated in a method validation study on roasted coffee, soluble coffee, coffee substitutes and coffee products with ranges from 53 μg/kg to 612,1 μg/kg.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Procedure
8.1 General
8.2 Preparation of the sample extract
8.3 Clean-up of the extracts
8.3.1 Carrez precipitation
8.3.2 Solid phase extraction
8.4 HPLC-MS/MS measurement
8.4.1 High-performance liquid chromatography (HPLC)
8.4.2 Identification and quantification by mass spectrometry (HPLC-MS/MS)
8.5 Measurement with GC-MS
8.5.1 Derivatization and sample preparation for gas chromatography
8.5.2 Gas chromatography
8.5.3 Identification and quantification by mass spectrometry
9 Calibration
9.1 General advice
9.2 Determination of linearity and definition of the working range
9.3 Calibration with internal standard solution
9.4 Determination of the laboratory specific recovery
10 Evaluation
10.1 Criteria for identification
10.2 Calculation and final results
11 Precision data
11.1 General
11.2 Repeatability
11.3 Reproducibility
11.4 Recovery
12 Measurement uncertainty
13 Test report
Annex A (informative) Performance characteristics
Annex B (informative) Examples of absorber materials
Annex C (informative) Examples of columns and analysis conditions
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Procedure
8.1 General
8.2 Preparation of the sample extract
8.3 Clean-up of the extracts
8.3.1 Carrez precipitation
8.3.2 Solid phase extraction
8.4 HPLC-MS/MS measurement
8.4.1 High-performance liquid chromatography (HPLC)
8.4.2 Identification and quantification by mass spectrometry (HPLC-MS/MS)
8.5 Measurement with GC-MS
8.5.1 Derivatization and sample preparation for gas chromatography
8.5.2 Gas chromatography
8.5.3 Identification and quantification by mass spectrometry
9 Calibration
9.1 General advice
9.2 Determination of linearity and definition of the working range
9.3 Calibration with internal standard solution
9.4 Determination of the laboratory specific recovery
10 Evaluation
10.1 Criteria for identification
10.2 Calculation and final results
11 Precision data
11.1 General
11.2 Repeatability
11.3 Reproducibility
11.4 Recovery
12 Measurement uncertainty
13 Test report
Annex A (informative) Performance characteristics
Annex B (informative) Examples of absorber materials
Annex C (informative) Examples of columns and analysis conditions
Bibliography