ISO 10273:2017
Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica
ISO 10273:2017 specifies a horizontal method for the detection of Y. enterocolitica associated with human disease. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of food production and food handling.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Principle
5.1 General
5.2 Direct plating from liquid enrichment medium
5.3 Enrichment in liquid enrichment medium and selective liquid enrichment medium
5.4 Plating out after enrichment and identification
5.5 Confirmation
6 Culture media and reagents
7 Equipment and consumables
8 Sampling
9 Preparation of test sample
10 Procedure (as shown in Annex A)
10.1 Test portion and initial suspension
10.2 Direct plating on selective agar
10.3 Enrichment
10.4 Plating out and incubation of plates
10.4.1 Plating from PSB and ITC by KOH treatment on CIN agar
10.4.2 Plating from PSB and ITC by KOH treatment on chromogenic agar (optional)
10.5 Identification of characteristic colonies
10.6 Confirmation
10.6.1 General
10.6.2 Selection of colonies for confirmation
10.6.3 Determination of pathogenic Yersinia species
10.6.4 Confirmation of pathogenic Y. enterocolitica
10.6.5 Interpretation of confirmation tests for Y. enterocolitica
10.6.6 Interpretation of confirmation tests for pathogenic Y. enterocolitica
10.7 Biotyping of Y. enterocolitica (optional)
10.7.1 General
10.7.2 Fermentation of xylose
10.7.3 Tween-esterase test
10.7.4 Fermentation of salicin (optional) and trehalose
10.7.5 Indole formation
10.7.6 Interpretation of biotyping tests
11 Expression of results
12 Performance characteristics of the method
12.1 Interlaboratory study
12.2 Sensitivity
12.3 Specificity
12.4 LOD50
13 Test report
14 Quality assurance
Annex A (normative) Diagrams of the procedures
Annex B (normative) Composition and preparation of culture media and reagents
Annex C (informative) Method validation studies and performance characteristics
Annex D (informative) Procedure for cold enrichment
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviated terms
5 Principle
5.1 General
5.2 Direct plating from liquid enrichment medium
5.3 Enrichment in liquid enrichment medium and selective liquid enrichment medium
5.4 Plating out after enrichment and identification
5.5 Confirmation
6 Culture media and reagents
7 Equipment and consumables
8 Sampling
9 Preparation of test sample
10 Procedure (as shown in Annex A)
10.1 Test portion and initial suspension
10.2 Direct plating on selective agar
10.3 Enrichment
10.4 Plating out and incubation of plates
10.4.1 Plating from PSB and ITC by KOH treatment on CIN agar
10.4.2 Plating from PSB and ITC by KOH treatment on chromogenic agar (optional)
10.5 Identification of characteristic colonies
10.6 Confirmation
10.6.1 General
10.6.2 Selection of colonies for confirmation
10.6.3 Determination of pathogenic Yersinia species
10.6.4 Confirmation of pathogenic Y. enterocolitica
10.6.5 Interpretation of confirmation tests for Y. enterocolitica
10.6.6 Interpretation of confirmation tests for pathogenic Y. enterocolitica
10.7 Biotyping of Y. enterocolitica (optional)
10.7.1 General
10.7.2 Fermentation of xylose
10.7.3 Tween-esterase test
10.7.4 Fermentation of salicin (optional) and trehalose
10.7.5 Indole formation
10.7.6 Interpretation of biotyping tests
11 Expression of results
12 Performance characteristics of the method
12.1 Interlaboratory study
12.2 Sensitivity
12.3 Specificity
12.4 LOD50
13 Test report
14 Quality assurance
Annex A (normative) Diagrams of the procedures
Annex B (normative) Composition and preparation of culture media and reagents
Annex C (informative) Method validation studies and performance characteristics
Annex D (informative) Procedure for cold enrichment
Bibliography