AS/NZS 4276.6:2007
Water microbiology - Method 6: Coliforms, Escherichia coli and thermotolerant coliforms - Determination of most probable number (MPN)
Combines the most probable number method for coliforms with that of thermotolerant coliforms and Escherichia coli to form a single method.
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AS/NZS 4276.6:2007 WATER MICROBIOLOGY - METHOD 6: COLIFORMS, ESCHERICHIA COLI AND THERMOTOLERANT COLIFORMS-DETERMINATION OF M
PREFACE
METHOD
1 SCOPE
2 REFERENCED DOCUMENTS
3 DEFINITIONS
3.1 Coliforms
3.2 Thermotolerant coliforms
3.3 Escherichia coli (E. coli)
4 PRINCIPLE
5 CULTURE MEDIA AND REAGENTS (See Appendix B)
6 APPARATUS
7 REFERENCE CULTURES
7.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309 or a culture traceable to any of these strains
7.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or NZRM 798 or a culture traceable to any of these strains
7.3 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
7.4 Use of reference cultures
8 SAMPLES
8.1 Laboratory samples
8.2 Dilutions
9 PROCEDURE
9.1 Presumptive test
9.2 Confirmatory tests
9.2.1 Coliforms
9.2.2 E. coli
9.2.3 Thermotolerant coliforms
10 DETERMINATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS
11 TEST REPORT
12 BIBLIOGRAPHY
APPENDIX A - FLOW DIAGRAM FOR COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS—MOST PROBABLE NUMBER
APPENDIX B - CULTURE MEDIA AND REAGENTS
B1 SCOPE
B2 IMPROVED FORMATE LACTOSE GLUTAMATE MEDIUM
B3 ORTHO-NITROPHENYL-β D-GALACTOPYRANOSIDE (ONPG) BROTH
B4 NUTRIENT AGAR
B5 MACCONKEY AGAR
B6 OXIDASE REAGENT (KOVACS’)
B7 EC-MUG BROTH
APPENDIX C - PERFORMANCE OF KOVACS’ OXIDASE TEST
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Related Information
Australia New Zealand Food Standards Code Standard 1.6.1
Ministry: Food Standards Australia New Zealand
Section: 4.1(b)
Citation context:...for packaged water, packaged ice or mineral water - the relevant method prescribed by Australian New Zealand Standard AS/NZS 4276.
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AS/NZS 4276.6:2007 WATER MICROBIOLOGY - METHOD 6: COLIFORMS, ESCHERICHIA COLI AND THERMOTOLERANT COLIFORMS-DETERMINATION OF M
PREFACE
METHOD
1 SCOPE
2 REFERENCED DOCUMENTS
3 DEFINITIONS
3.1 Coliforms
3.2 Thermotolerant coliforms
3.3 Escherichia coli (E. coli)
4 PRINCIPLE
5 CULTURE MEDIA AND REAGENTS (See Appendix B)
6 APPARATUS
7 REFERENCE CULTURES
7.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309 or a culture traceable to any of these strains
7.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or NZRM 798 or a culture traceable to any of these strains
7.3 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
7.4 Use of reference cultures
8 SAMPLES
8.1 Laboratory samples
8.2 Dilutions
9 PROCEDURE
9.1 Presumptive test
9.2 Confirmatory tests
9.2.1 Coliforms
9.2.2 E. coli
9.2.3 Thermotolerant coliforms
10 DETERMINATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS
11 TEST REPORT
12 BIBLIOGRAPHY
APPENDIX A - FLOW DIAGRAM FOR COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS—MOST PROBABLE NUMBER
APPENDIX B - CULTURE MEDIA AND REAGENTS
B1 SCOPE
B2 IMPROVED FORMATE LACTOSE GLUTAMATE MEDIUM
B3 ORTHO-NITROPHENYL-β D-GALACTOPYRANOSIDE (ONPG) BROTH
B4 NUTRIENT AGAR
B5 MACCONKEY AGAR
B6 OXIDASE REAGENT (KOVACS’)
B7 EC-MUG BROTH
APPENDIX C - PERFORMANCE OF KOVACS’ OXIDASE TEST
AS/NZS 4276.6:2007
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