AS/NZS 4276.22:2019
Water microbiology – Method 22: Water quality – Enumeration of Escherichia coli and coliform bacteria – Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016, MOD)
This standard specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria.
This method is not to be used for the enumeration of E. coli and coliform bacteria in surface or shallow well waters.
This standard is an adoption with national modifications and has been reproduced from ISO 9308-1:2014, Water quality — Enumeration of Escherichia coli and coliform bacteria, Part 1: Membrane filtration method for waters with low bacterial background flora and Amendment 1:2016.
In 2025, the P4828 Rejoining of Microbiology Standards committee was formed to consider this standard’s suitability (and the suitability of other Australian water microbiology standards) for rejoining as Australian/New Zealand standards.
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AS/NZS 4276.22:2019
SA/SNZ - Cover page
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus and glassware
6 Culture media and reagents
7 Sampling
8 Procedure
8.1 Preparation of the sample
8.2 Filtration
8.3 Incubation and differentiation
9 Expression of results
10 Test report
11 Quality assurance
11.1 General
11.2 Performance testing of Chromogenic Coliform Agar (CCA)
11.3 Performance testing of oxidase test
Annex A (informative) Further microbiological information on coliform bacteria
Annex B (normative) Composition and preparation of culture media and reagents
Annex C (informative) Performance characteristics
Bibliography
AMENDMENT 1
Appendix ZZ (normative) Variations to ISO 9308-1:2014 for Australian conditions
Appendix ZA (informative) Additional information for Australian conditions
Amendment control sheet
Previous versions
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AS/NZS 4276.22:2019
SA/SNZ - Cover page
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus and glassware
6 Culture media and reagents
7 Sampling
8 Procedure
8.1 Preparation of the sample
8.2 Filtration
8.3 Incubation and differentiation
9 Expression of results
10 Test report
11 Quality assurance
11.1 General
11.2 Performance testing of Chromogenic Coliform Agar (CCA)
11.3 Performance testing of oxidase test
Annex A (informative) Further microbiological information on coliform bacteria
Annex B (normative) Composition and preparation of culture media and reagents
Annex C (informative) Performance characteristics
Bibliography
AMENDMENT 1
Appendix ZZ (normative) Variations to ISO 9308-1:2014 for Australian conditions
Appendix ZA (informative) Additional information for Australian conditions
Amendment control sheet
Previous versions
AS/NZS 4276.22:2019
Online Library subscription information
Online library subscriptions provide a cost-effective solution for organisations of any size to access the latest standards, offering 24/7 access to a personalised catalogue. The service ensures subscribers have have the most up-to-date version of selected standards, incluing automatic updates for any amendments or versions. Pricing is dependent on the chosen standards and the number of concurrent users, with discounts applied from the standards PDF price. For customised subscriptions or further inquiries, contact the Online Library team.