ISO 21766:2021
Tobacco and tobacco products — Determination of tobacco-specific nitrosamines in tobacco products — Method using LC-MS/MS
This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in tobacco and the following tobacco products: cigarettes, cigars and smokeless tobacco products using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The TSNAs determined with this method are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Preparation
7.1 Preparation of glassware
7.2 Preparation of solutions
7.2.1 Extraction solution, 100 mM ammonium acetate in water
7.2.2 HPLC Mobile Phase A: Water, resistivity ≥ 18,2 MΩ·cm at 25 °C
7.2.3 HPLC Mobile Phase B: 0,1 % acetic acid in methanol
7.3 Preparation of standards
7.3.1 General
7.3.2 Preparation of internal standard solutions
7.3.3 Preparation of calibration standard solutions
8 Sampling
8.1 General
8.2 Sample preparation
8.3 Sample extraction
9 Sample analysis
9.1 General
9.2 Suggested HPLC parameters
9.3 MS/MS parameters
9.3.1 General
9.3.2 Quantification and qualification transitions
9.4 System suitability
9.5 Calibration
9.6 Calculation
10 Repeatability and reproducibility
11 Test report
Annex A (informative) Sample clean-up using solid phase extraction (SPE)
Annex B (informative) Examples of typical chromatograms
Bibliography
Previous versions
Keep me up-to-date
Sign up to receive updates when there are changes to this standard
Related Information
Similar Standards
-
BS 5202-1:2000
Methods for chemical analysis of tobacco and tobacco products, Determination of silicated residues insoluble in hydrochloric acid
-
BS 5202-10:2001
Methods for chemical analysis of tobacco and tobacco products, Determination of organochlorine pesticide residues (gas chromatographic method)
-
BS 5202-11:1983
Methods for chemical analysis of tobacco and tobacco products, Determination of dithiocarbamate residues
-
BS 5202-9:1992
Methods for chemical analysis of tobacco and tobacco products, Determination of alkaloid retention by filters of cigarettes (spectrometric method)
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Preparation
7.1 Preparation of glassware
7.2 Preparation of solutions
7.2.1 Extraction solution, 100 mM ammonium acetate in water
7.2.2 HPLC Mobile Phase A: Water, resistivity ≥ 18,2 MΩ·cm at 25 °C
7.2.3 HPLC Mobile Phase B: 0,1 % acetic acid in methanol
7.3 Preparation of standards
7.3.1 General
7.3.2 Preparation of internal standard solutions
7.3.3 Preparation of calibration standard solutions
8 Sampling
8.1 General
8.2 Sample preparation
8.3 Sample extraction
9 Sample analysis
9.1 General
9.2 Suggested HPLC parameters
9.3 MS/MS parameters
9.3.1 General
9.3.2 Quantification and qualification transitions
9.4 System suitability
9.5 Calibration
9.6 Calculation
10 Repeatability and reproducibility
11 Test report
Annex A (informative) Sample clean-up using solid phase extraction (SPE)
Annex B (informative) Examples of typical chromatograms
Bibliography