ISO 23305:2020
Fortified milk powders, infant formula and adult nutritionals — Determination of total biotin by liquid chromatography coupled with immunoaffinity column clean-up extraction
This document specifies a method for the quantitative determination of biotin and/or biocytin in fortified milk powders, infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using liquid chromatography coupled with immunoaffinity column clean-up extraction.
Precision data from an interlaboratory study is given in Annex B. A comparison between data obtained with the method in this document and EN 15607 is given in Annex C.
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
5.1 General
5.2 Reagent preparation
5.3 Standard preparation
5.4 Calculation of concentration
6 Apparatus
7 Procedure
7.1 Sample preparation
7.2 Chromatography
7.3 Quality control
8 Calculations
9 Precision
9.1 General
9.2 Repeatability
9.3 Reproducibility
10 Uncertainty of measurement
11 Limit of quantitation
Annex A (informative) Example chromatograms
Annex B (informative) Precision data
Annex C (informative) Comparison between this document and EN 15607
Bibliography
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
5.1 General
5.2 Reagent preparation
5.3 Standard preparation
5.4 Calculation of concentration
6 Apparatus
7 Procedure
7.1 Sample preparation
7.2 Chromatography
7.3 Quality control
8 Calculations
9 Precision
9.1 General
9.2 Repeatability
9.3 Reproducibility
10 Uncertainty of measurement
11 Limit of quantitation
Annex A (informative) Example chromatograms
Annex B (informative) Precision data
Annex C (informative) Comparison between this document and EN 15607
Bibliography