ISO 22634-2:2019
Cigarettes — Determination of benzo[a]pyrene in cigarette mainstream smoke using GC/MS — Part 2: Method using cyclohexane as extraction solvent
This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent.
This method is specified using ISO 3308 smoking parameters. This document provides an alternative method to that specified in ISO 22634-1, with a different clean-up, and a shorter total analytical run allowing a potential increase of sample throughput in comparison with ISO 22634-1.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Reagents
7 Standards
7.1 General
7.2 Primary B[a]P-d12 stock solution: 100 µg/ml
7.3 Secondary B[a]P-d12 spiking solution: 40 ng/ml
7.4 Primary B[a]P stock solution: 100 µg/ml
7.5 Secondary B[a]P stock solution: 1 000 ng/ml
7.6 Working standard solutions
7.7 Storage of standard solutions
8 Preparation of sample
8.1 Sampling
8.2 Smoking
8.3 Glass-fibre filter pad extraction
8.4 Sample clean-up
8.5 Blank solution
9 Determination
9.1 GC/MS operating conditions
9.2 Calibration
9.3 Determination of B[a]P
9.4 Calculation
10 Repeatability and reproducibility
11 Test report
11.1 General
11.2 Characteristic data about the cigarette
11.3 Data about sampling
11.4 Description of the test
11.5 Test results
Annex A (informative) Example of a chromatogram of a cigarette smoke extract
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
6 Reagents
7 Standards
7.1 General
7.2 Primary B[a]P-d12 stock solution: 100 µg/ml
7.3 Secondary B[a]P-d12 spiking solution: 40 ng/ml
7.4 Primary B[a]P stock solution: 100 µg/ml
7.5 Secondary B[a]P stock solution: 1 000 ng/ml
7.6 Working standard solutions
7.7 Storage of standard solutions
8 Preparation of sample
8.1 Sampling
8.2 Smoking
8.3 Glass-fibre filter pad extraction
8.4 Sample clean-up
8.5 Blank solution
9 Determination
9.1 GC/MS operating conditions
9.2 Calibration
9.3 Determination of B[a]P
9.4 Calculation
10 Repeatability and reproducibility
11 Test report
11.1 General
11.2 Characteristic data about the cigarette
11.3 Data about sampling
11.4 Description of the test
11.5 Test results
Annex A (informative) Example of a chromatogram of a cigarette smoke extract
Bibliography