ISO/TS 14101:2012
Surface characterization of gold nanoparticles for nanomaterial specific toxicity screening: FT-IR method
ISO/TS 14101:2012 provides guidelines for the identification of the surface bound molecules using FT-IR of dehydrated gold nanoparticle (AuNPs) films both before and after nanomaterial (NM) cytotoxicity testing.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Sample preparation mode
5.1 Removal of unbound molecules
5.2 Dehydration
5.3 Screening test for impurities in DW from sample tubes
6 FT-IR measurement procedure
6.1 General
6.2 ATR method
6.3 Transmission method
6.4 Determination of time required for complete purge
6.5 Linear range of IR band intensity versus concentration
6.6 LOD and LOQ determination
6.7 Repeatability determination
7 Application examples
7.1 Degrees of ligand exchange
7.2 Qualitative measurement of biomolecular binding
Annex A (informative) Case study for validation of ligand exchange
Annex B (informative) Case study for qualitative measurement of biochemical moieties binding to the AuNP surface
Annex C (informative) Selection guide for window materials
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Sample preparation mode
5.1 Removal of unbound molecules
5.2 Dehydration
5.3 Screening test for impurities in DW from sample tubes
6 FT-IR measurement procedure
6.1 General
6.2 ATR method
6.3 Transmission method
6.4 Determination of time required for complete purge
6.5 Linear range of IR band intensity versus concentration
6.6 LOD and LOQ determination
6.7 Repeatability determination
7 Application examples
7.1 Degrees of ligand exchange
7.2 Qualitative measurement of biomolecular binding
Annex A (informative) Case study for validation of ligand exchange
Annex B (informative) Case study for qualitative measurement of biochemical moieties binding to the AuNP surface
Annex C (informative) Selection guide for window materials
Bibliography