ISO 9936:2016
Animal and vegetable fats and oils — Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography
ISO 9936:2016 specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).
For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.
Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this International Standard.
NOTE A suitable method involving a cold saponification procedure is described in Annex B for information only.
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Preparation of calibration solutions
9.1.1 Stock calibration solutions
9.1.2 Standard solution
9.2 Optimization of working parameters
9.3 Preparation of test solution
9.4 Determination
10 Expression of results
11 Precision
11.1 Interlaboratory test
11.2 Repeatability
11.3 Reproducibility
12 Test report
Annex A (informative) Examples of chromatograms
Annex B (informative) Saponification
Annex C (informative) Results of interlaboratory tests
Bibliography
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Preparation of calibration solutions
9.1.1 Stock calibration solutions
9.1.2 Standard solution
9.2 Optimization of working parameters
9.3 Preparation of test solution
9.4 Determination
10 Expression of results
11 Precision
11.1 Interlaboratory test
11.2 Repeatability
11.3 Reproducibility
12 Test report
Annex A (informative) Examples of chromatograms
Annex B (informative) Saponification
Annex C (informative) Results of interlaboratory tests
Bibliography