ISO 4333:2022
Textiles — Determination of reduction activity of specific proteins derived from pollen, mite and other sources on textile products
This document specifies a test method for the determination of reduction activity of textile products against specific proteins which shows antigen-antibody reaction. This document only specifies the reduction activity against those proteins on the surface of textile products. It does not specify a testing method to evaluate the allergenic reaction against human beings.
Specific proteins which show antigen-antibody reaction are proteins derived from pollen, mite and other sources. Other specific proteins can be used after appropriate validation described in this document.
Enzyme-linked immunosorbent assay is used to quantify the amount of those proteins in this document.
This document is applicable to textile products include woven, knitted and nonwoven fabrics, fibres, yarns, braids, etc.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Specific proteins
6 Apparatus
7 Reagents and media
8 Preparation
8.1 Preparation of test specific protein suspension
8.2 Preparation of test specimens
8.3 Control test
8.3.1 General
8.3.2 Procedure of verification of the sensitivity of ELISA
8.3.3 Requirement for verification of the sensitivity of ELISA
9 Preparation of the specific protein calibration curve
10 Test procedure
10.1 Preparation of test specimen
10.2 Deposit of specific protein to the test specimens
10.3 Contacting time
10.4 Recovery of specific protein from the test specimens
11 Determination of the specific protein concentration
12 Calculation of the specific protein concentration and reduction rate
13 Repeatability and reproducibility
14 Test report
Annex A (informative) Specific protein derived from pollen and mite
Annex B (informative) ELISA assay reagents, reagents and buffer solutions
Annex C (informative) Enzyme-linked immunosorbent assay — Sandwich ELISA test procedure
Annex D (informative) Specific protein calibration curve
Annex E (informative) Recovery rate
Annex F (informative) Interlaboratory test result
Annex G (informative) Repeatability and reproducibility
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Specific proteins
6 Apparatus
7 Reagents and media
8 Preparation
8.1 Preparation of test specific protein suspension
8.2 Preparation of test specimens
8.3 Control test
8.3.1 General
8.3.2 Procedure of verification of the sensitivity of ELISA
8.3.3 Requirement for verification of the sensitivity of ELISA
9 Preparation of the specific protein calibration curve
10 Test procedure
10.1 Preparation of test specimen
10.2 Deposit of specific protein to the test specimens
10.3 Contacting time
10.4 Recovery of specific protein from the test specimens
11 Determination of the specific protein concentration
12 Calculation of the specific protein concentration and reduction rate
13 Repeatability and reproducibility
14 Test report
Annex A (informative) Specific protein derived from pollen and mite
Annex B (informative) ELISA assay reagents, reagents and buffer solutions
Annex C (informative) Enzyme-linked immunosorbent assay — Sandwich ELISA test procedure
Annex D (informative) Specific protein calibration curve
Annex E (informative) Recovery rate
Annex F (informative) Interlaboratory test result
Annex G (informative) Repeatability and reproducibility
Bibliography