ISO 23641:2021
Flexible cellular polymeric materials — Determination of antibacterial effectiveness
This document specifies a method of determining the antibacterial effectiveness of open-cell flexible cellular polymeric antibacterial treated materials, including their intermediate and final products.
This document is suitable for flexible cellular polymeric materials because the test procedure enables the test inoculum to efficiently contact with the surface of open cell in the flexible cellular polymeric materials.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Judgement criteria of antibacterial effectiveness
5 Test methods
5.1 Bacteria to be used for the tests
5.2 Reagents and materials
5.3 Equipment and apparatus
5.4 Sterilization methods
5.4.1 General
5.4.2 Dry-heat sterilization
5.4.3 High-pressure steam sterilization
5.4.4 Flame sterilization
5.5 Medium and buffer
5.5.1 General
5.5.2 1/500 nutrient broth medium (1/500 NB)
5.5.3 Slant culture medium
5.5.4 Standard nutrient agar medium (SA medium)
5.5.5 Phosphate-buffered physiological saline
5.6 Preservation of test strain
5.7 Procedure
5.7.1 General
5.7.2 Pre-culture of bacteria
5.7.3 Preparation of test specimens
5.7.4 Sterilization of specimens
5.7.5 Preparation of test inoculum
5.7.6 Inoculation onto the test specimens
5.7.7 Shaking incubation of the test specimens and the control group
5.7.8 Measurement of viable bacteria cell count
5.7.9 Notation of viable bacteria cell count
5.8 Expression of the results
5.8.1 Requirements for test validity
5.8.2 Calculation of the antibacterial activity value
6 Test report
Annex A (normative) Pour plate culture method
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Judgement criteria of antibacterial effectiveness
5 Test methods
5.1 Bacteria to be used for the tests
5.2 Reagents and materials
5.3 Equipment and apparatus
5.4 Sterilization methods
5.4.1 General
5.4.2 Dry-heat sterilization
5.4.3 High-pressure steam sterilization
5.4.4 Flame sterilization
5.5 Medium and buffer
5.5.1 General
5.5.2 1/500 nutrient broth medium (1/500 NB)
5.5.3 Slant culture medium
5.5.4 Standard nutrient agar medium (SA medium)
5.5.5 Phosphate-buffered physiological saline
5.6 Preservation of test strain
5.7 Procedure
5.7.1 General
5.7.2 Pre-culture of bacteria
5.7.3 Preparation of test specimens
5.7.4 Sterilization of specimens
5.7.5 Preparation of test inoculum
5.7.6 Inoculation onto the test specimens
5.7.7 Shaking incubation of the test specimens and the control group
5.7.8 Measurement of viable bacteria cell count
5.7.9 Notation of viable bacteria cell count
5.8 Expression of the results
5.8.1 Requirements for test validity
5.8.2 Calculation of the antibacterial activity value
6 Test report
Annex A (normative) Pour plate culture method