ISO 23293:2020
Milk-based infant formula powders — Quantification of whey protein content by sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE)
This document specifies a method for the determination of the whey to casein protein ratio, ranging from 20:80 to 80:20 in cow milk-based infant formula powders.
This method does not apply to the analysis of infant formulas containing hydrolysed protein or proteins from other sources (e.g. plants or milk from other mammals).
| Get this standard | Prices exclude GST | |
|---|---|---|
| PDF ( Single user document) |
$118.26 NZD
|
|
| HardCopy |
$152.17 NZD
|
|
| Networkable PDF |
Price varies
|
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
6 Apparatus
7 Procedure
7.1 Sample preparation
7.2 CGE analysis
7.3 Electropherogram processing
7.3.1 Electropherogram integration
7.3.2 Identification of the casein region
8 System suitability
9 Calculations and expression of results
9.1 Calculation of whey protein content
9.2 Expression of results
10 Precision
10.1 Interlaboratory tests
10.2 Infant formulas with a whey protein content of 40 % or more
10.2.1 Repeatability
10.2.2 Reproducibility
10.3 Infant formulas with a whey protein content below 40 %
10.3.1 Repeatability
10.3.2 Reproducibility
11 Test report
Annex A (informative) Example of a CGE analysis
Annex B (informative) Precision data
Bibliography
Keep me up-to-date
Register to receive notifications when updates are made to this standard.
Related Information
Similar Standards
-
BS 4285-2.5:1989
Microbiological examination for dairy purposes. Methods of general application for enumeration of microorganisms, Enumeration of bacteria by direct microscopic counts
-
BS 4285-3.11:1985
Microbiological examination for dairy purposes. Methods for detection and/or enumeration of specific groups of microorganisms, Detection and enumeration of faecal streptococci
-
BS 4285-3.2:1991
Microbiological examination for dairy purposes. Methods for detection and/or enumeration of specific groups of microorganisms, Enumeration of thermoduric bacteria
-
BS 4285-3.4.2:1989
Microbiological examination for dairy purposes. Methods for detection and/or enumeration of specific groups of microorganisms. Enumeration of lipolytic microorganisms, Tributyrin agar method
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
6 Apparatus
7 Procedure
7.1 Sample preparation
7.2 CGE analysis
7.3 Electropherogram processing
7.3.1 Electropherogram integration
7.3.2 Identification of the casein region
8 System suitability
9 Calculations and expression of results
9.1 Calculation of whey protein content
9.2 Expression of results
10 Precision
10.1 Interlaboratory tests
10.2 Infant formulas with a whey protein content of 40 % or more
10.2.1 Repeatability
10.2.2 Reproducibility
10.3 Infant formulas with a whey protein content below 40 %
10.3.1 Repeatability
10.3.2 Reproducibility
11 Test report
Annex A (informative) Example of a CGE analysis
Annex B (informative) Precision data
Bibliography