ISO 18465:2017
Microbiology of the food chain — Quantitative determination of emetic toxin (cereulide) using LC-MS/MS
ISO 18465:2017 describes the quantitative analysis of the emetic toxin cereulide using high performance liquid chromatography (HPLC) or ultra performance liquid chromatography (UHPLC) connected to a tandem mass spectrometer (LC-MS/MS).
ISO 18465:2017 is applicable to the analysis of the toxin in products intended for human consumption.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 General principle
5 Reagents
6 Apparatus and equipment
7 Procedure
7.1 Sample preparation
7.2 Standard preparation
7.3 LC-MS analysis
7.3.1 LC conditions
7.3.2 MS conditions and tuning parameters
7.3.3 Transitions (multiple reaction monitoring, MRM)
8 Calculation
9 Quality controls
10 Precision
10.1 General
10.2 Repeatability
10.3 Reproducibility
Annex A (informative) Results of the interlaboratory study
Annex B (informative) Possible transitions of cereulide in MS[3]
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 General principle
5 Reagents
6 Apparatus and equipment
7 Procedure
7.1 Sample preparation
7.2 Standard preparation
7.3 LC-MS analysis
7.3.1 LC conditions
7.3.2 MS conditions and tuning parameters
7.3.3 Transitions (multiple reaction monitoring, MRM)
8 Calculation
9 Quality controls
10 Precision
10.1 General
10.2 Repeatability
10.3 Reproducibility
Annex A (informative) Results of the interlaboratory study
Annex B (informative) Possible transitions of cereulide in MS[3]
Bibliography