ISO 17721-1:2021
Quantitative determination of antibacterial activity of ceramic tile surfaces — Test methods — Part 1: Ceramic tile surfaces with incorporated antibacterial agents
This document specifies test methods for evaluating the antibacterial activity of glazed and unglazed ceramic tile surfaces with incorporated antibacterial agents.
Secondary effects on ceramic tile surfaces due to antibacterial treatments, such as changes in chemical resistance, stain resistance or small colour differences, are not covered by this document. For chemical resistance refer to ISO 10545-13, for stain resistance refer to ISO 10545-14 and for colour differences refer to ISO 10545-16.
Any results obtained with this document will always refer to this document and the conditions used. Results obtained with this document indicate antibacterial activity under the specified experimental conditions used herein, and do not reflect activity under other circumstances where a variety of factors, such as temperature, humidity, different bacterial species, nutrient conditions, etc., are considered.
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Materials
4.1 Bacterial strains
4.2 Culture media and solutions
4.2.1 Non-ionic surfactant
4.2.2 Nutrient broth
4.2.3 1/500 nutrient broth
4.2.4 Nutrient agar
4.2.5 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP)
4.2.6 Phosphate buffer saline (PBS)
5 Test specimen
5.1 Size
5.2 Control
5.3 Precondition
5.4 Number of test specimen
6 Procedure
6.1 Preparation of test inoculum
6.2 Adhesive film
6.3 Inoculation of test specimens
6.4 Recovery of bacteria from non-treated control specimens at time t = 0 h
6.5 Incubation
6.6 Recovery of bacteria from post incubation test specimens
6.7 Enumeration of viable bacterial count from test specimen washout
7 Validation and calculations
7.1 General
7.2 Criteria for a valid test
7.3 Calculation of antibacterial activity
8 Test report
Bibliography
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Materials
4.1 Bacterial strains
4.2 Culture media and solutions
4.2.1 Non-ionic surfactant
4.2.2 Nutrient broth
4.2.3 1/500 nutrient broth
4.2.4 Nutrient agar
4.2.5 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP)
4.2.6 Phosphate buffer saline (PBS)
5 Test specimen
5.1 Size
5.2 Control
5.3 Precondition
5.4 Number of test specimen
6 Procedure
6.1 Preparation of test inoculum
6.2 Adhesive film
6.3 Inoculation of test specimens
6.4 Recovery of bacteria from non-treated control specimens at time t = 0 h
6.5 Incubation
6.6 Recovery of bacteria from post incubation test specimens
6.7 Enumeration of viable bacterial count from test specimen washout
7 Validation and calculations
7.1 General
7.2 Criteria for a valid test
7.3 Calculation of antibacterial activity
8 Test report
Bibliography