ISO 14371:2012
Water quality — Determination of fresh water sediment toxicity to Heterocypris incongruens (Crustacea, Ostracoda)
This International Standard specifies a method for the determination of lethal as well as sublethal effects of contaminated sediments on the ostracod crustacean Heterocypris incongruens after 6 d exposure.
The method is applicable not only to fresh water sediments, but also by extension to solid wastes and soils after addition of (uncontaminated) water.
The method can also be applied to chemicals or preparations which are spiked into a reference sediment.
This International Standard is not applicable to the testing of sediments from the estuarine or marine environment.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents, test organisms and media
7 Apparatus and material
8 Special precautions for sampling, transportation, storage and treatment of the sediment samples
9 Procedure
9.1 Hatching of the cysts
9.2 Pre-feeding of the ostracod neonates
9.3 Length measurement of the neonates
9.4 Addition of sediment and algal food to the test container
9.5 Transfer of the ostracod neonates into the test container
9.6 Incubation of the test system
9.7 Measurements
10 Reference test
11 Tests on pure chemicals or preparations
12 Validity criteria
13 Test report
Annex A (informative) Culturing of Heterocypris incongruens for cyst production
Annex B (informative) Precision data
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents, test organisms and media
7 Apparatus and material
8 Special precautions for sampling, transportation, storage and treatment of the sediment samples
9 Procedure
9.1 Hatching of the cysts
9.2 Pre-feeding of the ostracod neonates
9.3 Length measurement of the neonates
9.4 Addition of sediment and algal food to the test container
9.5 Transfer of the ostracod neonates into the test container
9.6 Incubation of the test system
9.7 Measurements
10 Reference test
11 Tests on pure chemicals or preparations
12 Validity criteria
13 Test report
Annex A (informative) Culturing of Heterocypris incongruens for cyst production
Annex B (informative) Precision data
Bibliography