ISO 14239:2017
Soil quality — Laboratory incubation systems for measuring the mineralization of organic chemicals in soil under aerobic conditions
ISO 14239:2017 specifies six suitable incubation systems for measuring the rates and extent of mineralization of organic compounds in soil by measurement of carbon dioxide (CO2) evolution. All incubation systems are applicable to soluble or insoluble compounds but choice of system depends on the overall purposes of the study.
ISO 14239:2017 does not apply to the use of such systems for material balance studies, which are often test-substance specific.
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Methods
4.1 General requirements
4.1.1 Soil collection and characterization
4.1.2 Test material
4.1.3 Incubation conditions
4.2 Choice of incubation systems
4.3 Flow-through system
4.3.1 Principle
4.3.2 Materials and reagents
4.3.3 Apparatus and glassware
4.3.4 Procedure
4.4 Soda-lime column system
4.4.1 Principle
4.4.2 Materials and reagents
4.4.3 Apparatus and glassware
4.4.4 Procedure
4.5 Biometer system
4.5.1 Principle
4.5.2 Materials and reagents
4.5.3 Apparatus and glassware
4.5.4 Procedure
4.6 Radiorespirometer
4.6.1 Principle
4.6.2 Materials and reagents
4.6.3 Apparatus, glass- and plastic-ware
4.6.4 Procedure
4.7 Microradiorespirometer
4.7.1 Principle
4.7.2 Materials and reagents
4.7.3 Apparatus, and plastic-ware.
4.7.4 Procedure
4.8 Miniaturized respirometer
4.8.1 Principle
4.8.2 Materials and reagents
4.8.3 Apparatus, and plastic-ware
4.8.4 Procedure
5 Calculation and expression of results
5.1 For unlabelled test materials
5.2 For 14C-labelled test materials
6 Test report
Bibliography
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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Methods
4.1 General requirements
4.1.1 Soil collection and characterization
4.1.2 Test material
4.1.3 Incubation conditions
4.2 Choice of incubation systems
4.3 Flow-through system
4.3.1 Principle
4.3.2 Materials and reagents
4.3.3 Apparatus and glassware
4.3.4 Procedure
4.4 Soda-lime column system
4.4.1 Principle
4.4.2 Materials and reagents
4.4.3 Apparatus and glassware
4.4.4 Procedure
4.5 Biometer system
4.5.1 Principle
4.5.2 Materials and reagents
4.5.3 Apparatus and glassware
4.5.4 Procedure
4.6 Radiorespirometer
4.6.1 Principle
4.6.2 Materials and reagents
4.6.3 Apparatus, glass- and plastic-ware
4.6.4 Procedure
4.7 Microradiorespirometer
4.7.1 Principle
4.7.2 Materials and reagents
4.7.3 Apparatus, and plastic-ware.
4.7.4 Procedure
4.8 Miniaturized respirometer
4.8.1 Principle
4.8.2 Materials and reagents
4.8.3 Apparatus, and plastic-ware
4.8.4 Procedure
5 Calculation and expression of results
5.1 For unlabelled test materials
5.2 For 14C-labelled test materials
6 Test report
Bibliography